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Effective part of thick-stalk corydalis pallida and preparation method and application of effective part

An effective part, the technology of Ethyl Acetate of Corydalis corydalis, which is applied in the field of plant extracts, can solve problems such as CDC25 enzymes that have not been seen yet, and achieve good inhibitory effect and good tumor inhibition rate.

Inactive Publication Date: 2016-12-07
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research and application of Corydalis chrysanthemum inhibiting the activity of CDC25 enzyme

Method used

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  • Effective part of thick-stalk corydalis pallida and preparation method and application of effective part

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiments 1 to 3 are specific implementations of the effective fractions of Corydalis chrysogenum and its preparation method of the present invention, wherein embodiment 1 is the best embodiment.

[0024] Example 1

[0025] The preparation method comprises the following steps: pulverize the whole plant of Corydalis viridifera, use an ethanol solution with a volume percentage concentration of 75% to 85% for ultrasonic extraction for 1 hour, then filter to obtain the ultrasonic extraction solution and filter residue, and reflux the filter residue for 3 times, each time for 0.5 hours , filtered to obtain the reflux extract, combined the ultrasonic extract and the reflux extract, concentrated and dried to obtain the extract; the extract was dispersed with distilled water to obtain the extract dispersion, and then sequentially used petroleum ether, ethyl acetate and n-butanol as solvent extraction For the extract dispersion, take the ethyl acetate extract and the n-butanol...

Embodiment 2

[0027] The preparation method comprises the following steps: pulverize the whole plant of Corydalis villosa, ultrasonically extract it with an ethanol solution with a concentration of 85% to 95% by volume for 3 hours, then filter to obtain the ultrasonic extract and filter residue, and reflux the filter residue for 3 times, 1 hour each time , filtered to obtain the reflux extract, combined the ultrasonic extract and the reflux extract, concentrated and dried to obtain the extract; the extract was dispersed with distilled water to obtain the extract dispersion, and then sequentially used petroleum ether, ethyl acetate and n-butanol as solvent extraction For the extract dispersion, take the ethyl acetate extract and the n-butanol extract to volatilize the solvents respectively to obtain the ethyl acetate part and the n-butanol part of the thick-stalked Corydalis; after the extract dispersion is extracted, the remaining liquid is concentrated and dried, Obtain the part of the aque...

Embodiment 3

[0029] The preparation method comprises the following steps: pulverize the whole plant of Corydalis viridifera, use an ethanol solution with a volume percentage concentration of 65%~75% for ultrasonic extraction for 0.5 hours, then filter to obtain the ultrasonic extraction solution and filter residue, and reflux the filter residue for extraction twice, each time for 2 hours , filtered to obtain the reflux extract, combined the ultrasonic extract and the reflux extract, concentrated and dried to obtain the extract; the extract was dispersed with distilled water to obtain the extract dispersion, and then sequentially used petroleum ether, ethyl acetate and n-butanol as solvent extraction For the extract dispersion, take the ethyl acetate extract and the n-butanol extract to volatilize the solvents respectively to obtain the ethyl acetate part and the n-butanol part of the thick-stalked Corydalis; after the extract dispersion is extracted, the remaining liquid is concentrated and ...

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Abstract

The invention discloses an effective part of thick-stalk corydalis pallida and a preparation method and application of the effective part, and belongs to the technical field of plant extract. The effective part is selected from the mixture of one or two or more of a thick-stalk corydalis pallida ethyl acetate part, a thick-stalk corydalis pallida n-butyl alcohol part and a thick-stalk corydalis pallida water phase extraction part in any mass ratio. The preparation method comprises the steps that whole thick-stalk corydalis pallida is smashed, an ethanol solution with the volume percentage concentration of 65-95% is added for reflux extraction, and the obtained extracting solution is concentrated and dried to obtain extract; distilled water is added in the extract for dispersion, extract dispersing liquid is obtained, petroleum ether, ethyl acetate and n-butyl alcohol are used as solvents in sequence for extracting the extract dispersing liquid, a solvent is volatilized away, and the thick-stalk corydalis pallida ethyl acetate part and the thick-stalk corydalis pallida n-butyl alcohol part are obtained; the extracted extract dispersing liquid is concentrated and dried to obtain the thick-stalk corydalis pallida water phase extraction part. The effective part of the thick-stalk corydalis pallida is applied in preparation of medicine for inhibiting CDC25 enzyme and can be used for preparing anti-tumor medicine.

Description

technical field [0001] The effective part of Corydalis chrysogenum and its preparation method and application belong to the technical field of plant extracts. Background technique [0002] Corydalis pachypoda (Franch.) Hand.-Mazz. is a plant of the genus Corydalis in the Papaaceae family, a perennial herb, produced in northwest Yunnan (Eryuan, Lijiang, Zhongdian, Weixi, Deqin) and southwest Sichuan Ministry (Muli, Mianning), Eastern Tibet (Cona), born in crevices or sandstones at an altitude of (2300~) 3000~4600 (~4700) meters. There may be distribution in Nepal [see: Flora of China. Volume 32. Beijing: Science Press, 1999, 32:304]. Corydalis thick stem is a commonly used traditional Chinese medicine, which is recorded in Chinese Materia Medica. It is used as a whole herb to clear away heat and detoxify, and treat carbuncles, sores and furuncles. Cure mainly: carbuncle sore furuncle. [0003] Malignant tumor is one of the major diseases that seriously endanger human healt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P35/00
CPCA61K36/66A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 林鹏程吴疆却生曾擎屹高世宝
Owner QINGHAI UNIV FOR NATITIES
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