Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed

A transgenic plant, grain weight technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of backwardness and inability to meet the needs of the people

Active Publication Date: 2016-12-07
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Soybean production lags far behind the pace of development

Method used

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  • Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed
  • Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed
  • Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed

Examples

Experimental program
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Effect test

Example Embodiment

[0087] Example 1. Expression Analysis of GmbZIP336 Gene in Different Developmental Processes of Soybean

[0088] 1. Taking the weight of soybean Williams 82 seeds as the standard (the specific standard is the weight percentage of the seeds in development that are full but not dehydrated), the soybean development process is divided into 7 stages, and the corresponding weight percentages of the 7 stages are 4%, 8%, 12%, 16%, 24%, 48%, 96% ( figure 1 ). The transcriptomes of seed development stages 3 (12%) and 5 (24%) were sequenced and compared, and transcription factors that were highly expressed in stage 6 were obtained, including Glyma13g39340. It was named GmbZIP336 gene.

[0089] 2. Respectively extract RNA from each developmental stage of soybean seed development, and reverse transcription to obtain cDNA; using cDNA as template, F and R were used to perform Real Time-PCR to detect the expression of GmbZIP336 gene, and soybean Tublin gene was used as the internal standard...

Example Embodiment

[0095] Example 2. Obtaining and phenotypic analysis of transgenic GmbZIP336 Arabidopsis

[0096] 1. Construction of overexpression vector using Gateway technology

[0097] 1. Acquisition of GmbZIP336 gene

[0098] The total RNA of soybean Williams 82 seedlings was extracted and reverse transcribed to obtain cDNA; GmbZIP336 gene was obtained by PCR amplification with cDNA as template and GmbZIP336-up and GmbZIP336-dp as primers. The primer sequences are as follows:

[0099] GmbZIP336-up: 5'-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGTCTCTCCAACAACCAAATCAA-3' (sequence 3);

[0100] GmbZIP336-dp: 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTTCCAGGATGCGCTTAGAGGCCTCC-3' (sequence 4).

[0101] 2. Press TA The kit (product of Invitogen Company, product catalog number 12536-017) has its own operating steps to combine the GmbZIP336 gene obtained above and the entry vector 8 / GW / TOPO( TA The BP recombination reaction is carried out to obtain the BP reaction product.

[0102] 3. Add 2.5 μl of the ...

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Abstract

The invention discloses an application of a soybean transcription factor GmbZIP336 and a soybean transcription factor GmbZIP336 encoding gene in the regulation of the grain weight of a seed. The thousand-seed weight of transgenic Arabidopis thaliana grains obtained by transfering GmbZIP336 gene to wild Arabidopis thaliana is substantially higher than that of wild Arabidopis thaliana, so a GmbZIP336 protein has an up-regulation effect on the grain weight of the seed, and over-expression of the GmbZIP336 encoding gene improves the seed weight of transgenic genes without influencing the normal growth of the plants. Experiments prove that the GmbZIP336 protein can be used as a target gene for improving the seed output of the plants, and has important application values in the field of plant breeding.

Description

technical field [0001] The invention relates to the application of soybean transcription factor GmbZIP336 and its encoding gene in regulating seed weight, and belongs to the field of biotechnology. Background technique [0002] Soybean is an important traditional crop with rich nutritional value. It is an important economic crop to provide grain, oil and feed. Soybean oil also has many uses in industrial production, such as biofuel, surfactant, softener, etc. China used to be the world's largest soybean producer. However, in recent years, my country's soybean production has only accounted for one-third of the demand, making China the world's largest soybean importer, with total imports accounting for half of the world's total soybean exports. Soybean production lags far behind the pace of development of other domestic food crops and cannot meet national needs. In the past 50 years, soybean production has only increased by 62%, while other food crops have increased by more t...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10C12N15/11A01H5/00
Inventor 张劲松陈受宜陆翔马彪张万科林晴何锶洁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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