A kind of pectin, its composition, and its application in antitumor
A composition and pectin technology, which can be used in antitumor drugs, drug combinations, medical preparations containing active ingredients, etc., can solve the problems of benign and malignant tumor identification and difficult postoperative recurrence.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Example 1: Preparation and structural characterization of pectin
[0041] 1) Pectin extraction
[0042] (1) Orange peel pretreatment: citrus raw materials, cleaned and minced, dried at 60-80°C, crushed, and passed through 40-80 mesh.
[0043] (2) Pigment removal: the pretreated orange peel was leached at 1:20 (mass ratio) with 70% ethanol at 60-80°C for 2 hours, press-filtered to obtain solids, and the extraction was repeated 3 times.
[0044] (3) Extract pectin by acid method: add a certain volume of water to the pretreated solid matter according to the solid-liquid ratio of 1:20 (mass ratio), and then adjust the pH of the solution to 2-4 with 5% hydrochloric acid solution, React at 80°C for 2 hours, filter, and the filtrate is the pectin extract.
[0045] (4) Concentration of the pectin extract: Concentrate the pectin extract under reduced pressure under vacuum at a concentration temperature of 55°C to 1 / 4 to 1 / 3 of the original volume.
[0046] (5) Ethanol-precipi...
Embodiment 2
[0068] Example 2: Fluorescence-labeled pectin can be intestinally absorbed by human intestinal mucosal epithelial HIMC cells and HT-29 colon cancer cells
[0069] 1) Fluorescent labeling method of pectin and preparation of fluorescently labeled pectin
[0070] Prepare saturated CNBr solution and 0.2M NaOH solution.
[0071] Weigh 20mg of pectin and dissolve it in 10ml of distilled water, stir well to dissolve. Add 4ml CNBr solution, add 25μl NaOH solution each time, keep adding (ensure pH>10), continue for 7min, and observe the solution change, if precipitation occurs, the experiment fails and needs to be repeated.
[0072] Dialyze the reaction solution in the previous step against distilled water for 1-2 days, and replace the dialyzed external solution every 2-3 hours.
[0073] Concentrate the solution in the bag to dryness, add 0.2M Na at pH=8 2 B 4 o 7 .12H 2 O (borax) 2-3ml After dissolving the concentrate, add a small amount of fluorescein (about 1 mg) and react ove...
Embodiment 3
[0079] Example 3: Pectin inhibits the proliferation of colon cancer cell line HT-29
[0080] 1) Cell culture
[0081] HT29 colon cancer cells (purchased from ATCC) were incubated with DMEM high-glucose medium (containing 10% fetal bovine serum) at 37°C, saturated humidity, 5% CO 2 cultured in a cell culture incubator, and the medium was changed once every 2-3 days. When the confluence of the cells reached 100%, they were digested with 0.25% trypsin (Invitrogen), and subcultured at 1:2.
[0082] 2) MTT determination
[0083] Digested cells were seeded into 96-well cell culture plates at 5000 cells / well, with a volume of 100 μL per well. 37°C, saturated humidity, 5% CO 2 cultured under conditions until the cells adhered to the wall.
[0084] After the prepared pectin was dissolved in DMEM high-sugar complete medium to prepare pectin solution (2500, 200, 100, 50, 25 μg / ml), the supernatant of cells cultured overnight in the 96-well cell culture plate was sucked off, and adde...
PUM
| Property | Measurement | Unit |
|---|---|---|
| polydispersity index | aaaaa | aaaaa |
| polydispersity index | aaaaa | aaaaa |
| polydispersity index | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More