Double-layer microfluidic chip for tumor cell sorting

A technology of microfluidic chips and tumor cells, applied in the field of microfluidic chips, can solve the problems of inability to perform sorting operations, blockage of blood cells on the chip, slow sorting speed, etc., and achieve good later detection and tumor-specific research and operation The effect of simplicity and detection speed

Active Publication Date: 2016-12-07
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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Problems solved by technology

The disadvantage of this method is that the sorting speed is too slow, the efficiency is low, and the chip is easily blocked by blood cells, which makes the sorting operation impossible.

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  • Double-layer microfluidic chip for tumor cell sorting

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Embodiment Construction

[0025] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0026] Such as figure 1 As shown, the double-layer microfluidic chip for tumor cell sorting in this embodiment includes a first chip 1 and a second chip 2 located below the first chip 1, the surface of the first chip 1 has a sample channel, and The surface of the second chip 2 opposite to the sample channel has a buffer channel 11 , and the buffer channel 11 has a buffer inlet 19 and a buffer outlet 20 .

[0027] The first chip 1 has a sample inlet 4, and the sample channel includes several groups of sample channel groups 21, and each group of sample channel groups 21 includes several blood flow channels 23, and the sample inlet 4 connects with each blood flow channel through the sample guide channel group 22. 23 is connected to the inlet end; the first chip 1 is provided with a waste liquid outlet 3 corresponding to each group of sample chan...

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Abstract

The invention discloses a double-layer microfluidic chip for tumor cell sorting, comprising a first chip and a second chip positioned below the first chip; the surface of the first chip has a sample passage, the surface of the first chip facing the sample passage has a buffer passage, and the sample passage is cross-communicated with the buffer passage. Compared with CellSearch detection method, the technique of using the double-layer microfluidic chip to analyze tumor cells can be finished continuously in one step with no need for multiple steps and is simple to perform, detection speed is high, the purity of enriched CTCs is higher, no antibody is used, and CTCs obtained by the technique have high cell activity and may be better used in subsequent detection and tumor specificity research.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a double-layer microfluidic chip, and further relates to a double-layer microfluidic chip for tumor cell sorting. Background technique [0002] Circulating tumor cells (CTCs) are cancer cells that break away from the primary lesion, invade and enter the circulatory system. Research hotspots in oncology. However, the number of CTCs in the blood of tumor patients is very rare, with only 1 to 100 CTCs per milliliter of blood, and millions of white blood cells and more red blood cells per milliliter of blood. It is very difficult to select CTCs with high purity. [0003] Microfluidic chip technology integrates the sample preparation, chemical reaction, separation and enrichment, detection methods and other operating units involved in the fields of biology and chemistry into a glass slide of several square centimeters. Compared with conventional enrichment techniques, it has the characteristi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
Inventor 韩乙丁舒伟良陈艳
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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