Method for layered culture and enrichment of arbuscular mycorrhizal fungi spores

A technology of arbuscular mycorrhizal fungi and spores, which is applied in the field of microorganisms, can solve the problem that it is not easy to enrich a large number of arbuscular mycorrhizal fungal spores, and achieve the effect of simple use and high feasibility

Active Publication Date: 2016-12-07
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the defect that it is difficult to enrich a large number of arbuscular mycorrhizal fungal spores

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  • Method for layered culture and enrichment of arbuscular mycorrhizal fungi spores

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[0014] The present invention will be further described in detail below with reference to specific embodiments and drawings. The present embodiment is only for explaining the present invention and not limiting the present invention:

[0015] a.First use clean river sand with a particle size of 2mm as the lower matrix (6) to fill one-third of the culture vessel, and then fill 1cm thick clean river sand with a particle size of 4mm as the middle matrix (5) ;

[0016] b. Then place the isolation net (4) with a frame of 45um on the middle matrix (5), and the periphery of the frame is tightly attached to the inner wall of the culture container (1), so that the upper root system cannot be cultivated from the frame The container (1) fits through;

[0017] c. Mix the collected rhizosphere soil of fresh field host plants with 2mm clean river sand as the upper substrate (3) and fill it on the isolation net (4), and then plant the sterile host plant plants on the upper substrate (3) Inside;

[0...

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Abstract

Disclosed is a method for layered culture and enrichment of arbuscular mycorrhizal fungi spores. The method comprises the steps that a lower layer substrate, a middle layer substrate, an isolation net and an upper layer substrate are placed inside a culture container in a layered mode, sterile host plants are planted into the upper layer substrate; the culture container is placed into a container tray, and a drainage and suction opening is formed in the bottom of the culture container; a low-phosphorous nutrient solution is poured on the plant planting layer, a high-phosphorous nutrient solution is poured into the container tray, due to the fact that the culture container is divided into three layers and the middle layer is adopted as a buffer layer, the basic plant growth requirement is met by pouring the low-phosphorous nutrient solution into the plant planting layer, the lower layer substrate is subjected to siphoning of the high-phosphorous nutrient solution so as to maintain the lower layer high-phosphorous environment, meanwhile middle layer large particles cut off the siphoning from the bottommost layer to the plant planting layer, arbuscular mycorrhizal fungi certainly will enter the lower layer in order to help a host to absorb phosphorous, and a larger number of spores are produced on the lower layer when the host plant is about to die, so that a large number of arbuscular mycorrhizal fungi spores are obtained on the bottommost layer for single pore propagation and identification.

Description

technical field [0001] The invention relates to a method for enriching arbuscular mycorrhizal fungi, in particular to a method for layered culture and enrichment of arbuscular mycorrhizal fungal spores, belonging to the field of microorganisms. Background technique [0002] Arbuscular mycorrhizal fungi are a class of fungi that are widely symbiotic with plants in soil. There are many types of them, and they have been separately planned as Glomeromycota. Because of the mutualistic symbiotic relationship between it and plants, people have extensively studied its interaction mechanism, and collected bacterial species and strains from different regions to discover strains or new species with different characteristics. However, due to the small number of active spores of this type of fungus in the wild environment, it is difficult to isolate and identify them. Therefore, the enrichment of such fungi is relatively difficult. The traditional enrichment method is to use the origina...

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
Inventor 唐明胡文涛陈辉
Owner SOUTH CHINA AGRI UNIV
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