Method for fermentation production of cephalosporin C

A fermentation method and cephalosporin technology, which are applied in the field of fermentation to produce cephalosporin C, can solve the problems of low cephalosporin C production, achieve good industrial application prospects, increase single batch production, and reduce costs

Active Publication Date: 2016-12-07
上海锐康生物技术研发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The yield of cephalosporin C prepared by the fermentation method reported in the existing literature is low, and it is difficult to meet the requirements of industrial application well.

Method used

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  • Method for fermentation production of cephalosporin C

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 The method for fermentation production of cephalosporin C of the present invention

[0037] 1. Fermentation method

[0038] (1) Preparation of primary seeds

[0039] Take the slant surface of the eggplant bottle of Cephalosporium acremonium with the deposit number ATCC36225, and prepare a bacterial suspension of 25% (mg / mL). According to the inoculum of 1%, it was inoculated into the seed medium, and cultured at pH 7.0 and 27°C for 50h.

[0040] First-class seed medium: corn steep liquor 20g, soybean oil 35mL, glucose 13g, sucrose 10g, antifoaming agent 0.5g, calcium carbonate 1g.

[0041] (2) Preparation of secondary seeds

[0042] The first-class seeds were inoculated into the second-class seed medium according to 8% of the inoculum, and cultured at pH 6.5 and 27°C for 50h.

[0043] Secondary seed medium: corn steep liquor 16g, soybean oil 40mL, glucose 6g, antifoaming agent 0.5g, calcium carbonate 4g, calcium sulfate 5g, peanut flour 4g, soybean flou...

Embodiment 2

[0066] Embodiment 2 The method for producing cephalosporin C by fermentation of the present invention

[0067] 1. Fermentation method

[0068] (1) Preparation of primary seeds

[0069] Take the slant of the eggplant bottle of Cephalosporium acremonium with preservation number ATCC36225, prepare 25% (mg / mL) bacterial suspension, inoculate it into the seed medium according to the inoculation amount of 1%, and cultivate at pH 7.0 and 27°C for 50h.

[0070] First-class seed medium: corn steep liquor 20g, soybean oil 35mL, glucose 13g, sucrose 10g, antifoaming agent 0.5g, calcium carbonate 1g.

[0071] (2) Preparation of secondary seeds

[0072] The first-class seeds were inoculated into the second-class seed medium according to 8% of the inoculum, and cultured at pH 6.5 and 27°C for 50h.

[0073] Secondary seed medium: corn steep liquor 16g, soybean oil 40mL, glucose 6g, antifoaming agent 0.5g, calcium carbonate 4g, calcium sulfate 5g, peanut flour 4g, soybean flour 4g.

[007...

Embodiment 3

[0091] Embodiment 3 The method for producing cephalosporin C by fermentation of the present invention

[0092] 1. Fermentation method

[0093] (1) Preparation of primary seeds

[0094] Take the slant of the eggplant bottle of Cephalosporium acremonium with preservation number ATCC36225, prepare the bacterial suspension 25% (mg / mL), inoculate it into the seed medium according to the inoculation amount of 1%, and cultivate at pH 7.0 and 27 ℃ for 50h.

[0095] First-class seed medium: corn steep liquor 20g, soybean oil 35mL, glucose 13g, sucrose 10g, antifoaming agent 0.5g, calcium carbonate 1g.

[0096] (2) Preparation of secondary seeds

[0097] The first-class seeds were inoculated into the second-class seed medium according to 8% of the inoculum, and cultured at pH 6.5 and 27°C for 50h.

[0098] Secondary seed medium: corn steep liquor 16g, soybean oil 40mL, glucose 6g, antifoaming agent 0.5g, calcium carbonate 4g, calcium sulfate 5g, peanut flour 4g, soybean flour 4g.

...

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Abstract

The invention provides a method for fermentation production of cephalosporin C. The method comprises a step a of taking cephalosporins acremonium, and preparing primary seeds and secondary seeds; a step b of taking the secondary seeds prepared through the step a, inoculating the secondary seeds to a cephalosporins acremonium fermentation medium to perform fermentation, performing discharging after fermentation for 50-115 h, and re-cultivating the discharged liquid; a step c of taking the fermentation broth and the discharged culture liquid obtained through the step b, performing separation and purification, and obtaining cephalosporin C. According to the fermentation method, the single batch yield of cephalosporin C can be remarkably improved, the cost is greatly reduced, and the industrial application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to a method for producing cephalosporin C by fermentation. Background technique [0002] Cephalosporin C (English full name Cephalosporin C, abbreviated as CPC) is a class of β-lactam antibiotics produced by Cephalosporium acremonium, the molecular formula is: C 16 H 21 N 3 O 8 S, the molecular weight is 415.4, and the structural formula is: [0003] [0004] The structure of cephalosporin C is similar to that of penicillin, the difference is that the parent nucleus of cephalosporin C is 7-aminocephalosporanic acid (7-ACA), while the parent nucleus of penicillin is 6-aminopenicillic acid. It is precisely because of this structural difference that cephalosporin C has stronger stability. Cephalosporins C are widely used in clinical anti-inflammatory and antibacterial drugs due to their broad antibacterial spectrum and low incidence of adverse reactions, and are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P35/06C12R1/645
CPCC12P35/06
Inventor 王孟强许会卿党建宁王雁庆孙磊磊邓旭衡刘思川葛均友万阳浴
Owner 上海锐康生物技术研发有限公司
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