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A kind of method for fermentation production of cephalosporin c

A technology of cephalosporin and cephalosporin acremonium, which is applied in the field of microbial fermentation, can solve the problems of low yield of cephalosporin C and achieve the effects of good industrial application prospects, cost reduction, and single-batch yield increase

Active Publication Date: 2020-06-16
SHANGHAI RECOM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The yield of cephalosporin C prepared by the fermentation method reported in the existing literature is low, and it is difficult to meet the requirements of industrial application well.

Method used

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  • A kind of method for fermentation production of cephalosporin c

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 The method of fermentative production cephalosporin C of the present invention

[0037] 1. Fermentation method

[0038] (1) Preparation of primary seeds

[0039] Take the slant of the Cephalosporium acremonium eggplant bottle with the preservation number ATCC36225, and prepare a 25% (mg / mL) bacterial suspension. Inoculate into the seed medium according to the inoculum amount of 1%, and cultivate at pH 7.0 and 27° C. for 50 h.

[0040] Primary seed medium: 20g corn steep liquor, 35mL soybean oil, 13g glucose, 10g sucrose, 0.5g defoamer, 1g calcium carbonate.

[0041] (2) Preparation of secondary seeds

[0042] The primary seeds were inoculated in the secondary seed medium according to the inoculum amount of 8%, and cultured at pH 6.5 and 27° C. for 50 h.

[0043] Secondary seed medium: 16g of corn steep liquor, 40mL of soybean oil, 6g of glucose, 0.5g of defoamer, 4g of calcium carbonate, 5g of calcium sulfate, 4g of peanut powder, and 4g of soybean powd...

Embodiment 2

[0066] Embodiment 2 The method of fermentative production cephalosporin C of the present invention

[0067] 1. Fermentation method

[0068] (1) Preparation of primary seeds

[0069] Take the slant of the Cephalosporium acremonium eggplant bottle with the preservation number ATCC36225, prepare a 25% (mg / mL) bacterial suspension, inoculate it into the seed medium according to the inoculum size of 1%, and cultivate it at a pH of 7.0 and 27°C for 50 hours.

[0070] Primary seed medium: 20g corn steep liquor, 35mL soybean oil, 13g glucose, 10g sucrose, 0.5g defoamer, 1g calcium carbonate.

[0071] (2) Preparation of secondary seeds

[0072] The primary seeds were inoculated in the secondary seed medium according to the inoculum amount of 8%, and cultured at pH 6.5 and 27° C. for 50 h.

[0073] Secondary seed medium: 16g of corn steep liquor, 40mL of soybean oil, 6g of glucose, 0.5g of defoamer, 4g of calcium carbonate, 5g of calcium sulfate, 4g of peanut powder, and 4g of soybea...

Embodiment 3

[0091] Embodiment 3 The method of fermentative production cephalosporin C of the present invention

[0092] 1. Fermentation method

[0093] (1) Preparation of primary seeds

[0094] Take the slant of the Cephalosporium acremonium eggplant bottle with the preservation number ATCC36225, prepare a 25% (mg / mL) bacterial suspension, inoculate it into the seed medium according to the inoculum size of 1%, and cultivate it at a pH of 7.0 and 27°C for 50 hours.

[0095] Primary seed medium: 20g corn steep liquor, 35mL soybean oil, 13g glucose, 10g sucrose, 0.5g defoamer, 1g calcium carbonate.

[0096] (2) Preparation of secondary seeds

[0097] The primary seeds were inoculated in the secondary seed medium according to the inoculum amount of 8%, and cultured at pH 6.5 and 27° C. for 50 h.

[0098] Secondary seed medium: 16g of corn steep liquor, 40mL of soybean oil, 6g of glucose, 0.5g of defoamer, 4g of calcium carbonate, 5g of calcium sulfate, 4g of peanut powder, and 4g of soybea...

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Abstract

The invention provides a method for fermentation production of cephalosporin C. The method comprises a step a of taking cephalosporins acremonium, and preparing primary seeds and secondary seeds; a step b of taking the secondary seeds prepared through the step a, inoculating the secondary seeds to a cephalosporins acremonium fermentation medium to perform fermentation, performing discharging after fermentation for 50-115 h, and re-cultivating the discharged liquid; a step c of taking the fermentation broth and the discharged culture liquid obtained through the step b, performing separation and purification, and obtaining cephalosporin C. According to the fermentation method, the single batch yield of cephalosporin C can be remarkably improved, the cost is greatly reduced, and the industrial application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for producing cephalosporin C by fermentation. Background technique [0002] Cephalosporin C (English full name is Cephalosporin C, abbreviated as CPC), is a class of β-lactam antibiotics produced by Cephalosporium acremonium, and its molecular formula is: C 16 h 21 N 3 o 8 S, the molecular weight is 415.4, and the structural formula is: [0003] [0004] The structure of cephalosporin C is similar to that of penicillin. The difference is that the core of cephalosporin C is 7-aminocephalosporanic acid (7-ACA), while the core of penicillin is 6-aminopenicillanic acid. It is because of this structural difference that cephalosporin C has stronger stability. Cephalosporins C are anti-inflammatory and antibacterial drugs widely used clinically because of their wide antibacterial spectrum and low incidence of adverse reactions, and are also t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P35/06C12R1/645
CPCC12P35/06
Inventor 王孟强许会卿党建宁王雁庆孙磊磊邓旭衡刘思川葛均友万阳浴
Owner SHANGHAI RECOM BIOTECHNOLOGY CO LTD
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