Preparation method of pesticide residue rapid detection kit, stabilizer and cholinesterase enzyme powder

Abstract

The invention discloses a pesticide residual toxicity rapid detection kit, a preparation method of a stabilizer and a preparation method of cholinesterase powder. The pesticide residual toxicity rapid detection kit comprises cholinesterase powder, a stabilizer, a substrate, a color developing agent and a buffer agent. The cholinesterase powder is a compound of cholinesterase and the stabilizer. The cholinesterase is a mixture of acetylcholin esterase and butyrylcholine esterase. The stabilizer is a compound of hydroxypropyl methyl cellulose, cane sugar, n-octyl alcohol, gelatin, glutaraldehyde, bovine serum albumin and sodium benzoate. The color developing agent is di-sulfur dinitrobenzoic acid, and the buffer agent is composed of disodium hydrogen phosphate and sodium dihydrogen phosphate. The stabilizer is added into the cholinesterase to be combined with the cholinesterase to obtain cholinesterase powder, the normal temperature storage time is greatly prolonged, normal temperature transportation and storage can be performed within the service life, the enzymatic activity is high, the stability is good, and detection is convenient.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to a rapid detection kit for pesticide residues, a method for preparing a stabilizer and a method for preparing cholinesterase enzyme powder. Background technique [0002] With the rapid development of agricultural production and construction, the widespread use of pesticides can effectively fight against pests and diseases and ensure the increase of grain and vegetable income. However, at the same time, a series of food safety problems caused by excessive use and residues of pesticides are also attracting more and more attention. In order to ensure that people's health and lives and properties are not affected, it is necessary to detect pesticide residues in food. [0003] At present, the techniques for detecting pesticide residues are divided into two categories: one is quantitative detection technology, and the other is qualitative rapid detection technology. ...

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Problems solved by technology

[0005] Although the application of the above-mentioned invention patents has solved the problem of organic phosphorus and carbamate pesticide residues in some agricultural products in a small area, the above-mentioned patented products have the following disadvantages: cholinesterase is easy to lose activity at room temperature and cannot be processed at room temperature. For transportation and storage, it is necessary to add additional refrigeration equipment to ensure that the inactivation rate of cholinesterase is reduced during transportation and storage, which virtually increases additional transportation and storage costs

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