A flow cytometry chip

Abstract

The invention discloses a fluidic cell detection chip. A first protection film, a first sealing film, a microfluidic chip and a second sealing film which are strippable are sequentially composited from top to bottom; the first sealing film is provided with at least four electrode holes, one sample adding hole and one vent hole; a liquid storage tank, a sample adding tank, a detection channel and a waste liquid tank which are communicated and at least two detection holes located at the two ends of the detection channel are arranged on the microfluidic chip in the use direction of the chip from top to bottom; the setting positions of the four electrode holes correspond to the liquid storage tank, two detection holes and an inlet of the waste liquid tank respectively; the setting position of the sample adding hole corresponds to the sample adding tank, and the setting position of the vent hole corresponds to the waste liquid tank. According to the fluidic cell detection chip, power for liquid flow is provided by means of a gravity principle, and therefore a drive does not need to be additionally arranged; the chip is small in size and low in cost, and miniaturization and energy conservation of equipment used in the detection process are achieved; meanwhile, the electrode holes are arranged for sampling detection, and the accuracy of a detection result is guaranteed.

Description

Technical field [0001] The invention relates to a flow cell detection chip. Background technique [0002] Flow particle detection is a technique for quantitative analysis and sorting of particles in liquid one by one. It can measure particle size, shape, concentration, activity, etc. It is widely used in hematology, immunology, molecular biology and other disciplines. Applications. The Coulter principle used in its detection refers to: when the particles suspended in the electrolyte pass through the small hole with the electrolyte, it replaces the same volume of the electrolyte, resulting in a constant current design circuit between the two electrodes inside and outside the small hole. The resistance changes instantaneously and generates a potential pulse. The size and frequency of the pulse signal are proportional to the size and number of particles. Sample focusing is the key technology of flow particle detection. Current detection uses chips that focus on the sample liquid b...

AI Technical Summary

Problems solved by technology

Sample focusing is the key technology of flow particle detection. In the current detection, chips that focus on the sample liquid through external force are used. This type of chip integrates the detection electrodes in the chip, which has a large volume and is provided by negative pressure during use. Power, and it is necessary to add buffer to the chip when using it. How and how much buffer is added will also affect the use of the chip

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