A biosensing calibration method, calibration system and disease detection system

Abstract

The present application discloses a biosensing calibration method, a calibration system, and a disease detection system, wherein the biosensing calibration method immobilizes a layer of the first antibody of the biomarker on the surface of the optical fiber to make part of the When the optical fiber passes through the sample to be tested, the antigen in the sample to be tested can be captured by the first antibody; then the second antibody of the biomarker to be tested is immobilized on the surface of the metal nanoparticles, and the metal nanoparticles are mixed into the In the tested sample, due to the specific recognition of the second antibody, the metal nanoparticles can be combined with the antigen in the tested sample through the second antibody; using the biosensing calibration method can Realize the detection of the antigen in the tested sample. Since the extinction cross section of metal nanoparticles is much larger than that of traditional fluorescent molecules, compared with traditional fluorescent labeling methods, the biosensing calibration method has a higher detection sensitivity for the antigen.

Description

technical field [0001] The present application relates to the technical field of biosensing, and more specifically, relates to a biosensing calibration method, a calibration system and a disease detection system. Background technique [0002] At present, in the medical field, the cost of disease detection accounts for a large part of patients' medical expenses. How to reduce the cost of disease detection has become one of the directions of researchers' efforts. Biosensing technology has greatly reduced the amount of detection reagents and greatly reduced the manufacturing cost of detection instruments. Therefore, biosensing technology has become a key research direction in the field of medical detection. [0003] Fluorescent labeling is a widely used biosensing technology at present. Its specific principle is to label fluorescein on known antibodies or antigen molecules. After the antibody is combined to form a complex, a certain amount of fluorescein is carried on the comp...

AI Technical Summary

Problems solved by technology

However, the problem with the fluorescent labeling method is that the fluorescein in the complex will inevitably affect the properties of the antigen to be tested or the antibody to be tested, and this effect may cause damage to the carrier of the antigen to be tested or the antibody to be tested ; and the fluorescein used for labeling may also produce fluorescence quenching, so that the fluorescent complex cannot be seen under the fluorescence microscope, leading to misdiagnosis

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