Low abundance hormone detection method and device

Abstract

The invention discloses a low abundance hormone detection method and device. A device for executing detection is provided with a multi-layer structure. Electrophoresis through hole regions of each layer structure are communicated to form an electrophoresis channel. The method comprises the steps that a trailing electrolyte and to-be-detected liquid are dropped on the surface of a top layer of the device; a leading electrolyte is dropped on the surface of a bottom layer of the device; electrophoresis is started by applying direct current voltage between the top layer and the bottom layer, so that the trailing electrolyte and the to-be-detected liquid are migrated in the direction of the bottom layer along the electrophoresis channel, and the leading electrolyte is migrated in the direction of the top layer along the electrophoresis channel; during electrophoresis, when to-be-detected hormone in the to-be-detected liquid is migrated and gathered on an enrichment layer of the device, and electrophoresis is stopped by removing the direct current voltage applied between the top layer and the bottom layer; the to-be-detected hormone gathered on the enrichment layer is moved to a detection region of a detection layer of the device, and electrochemical detection is performed on the detection region, and the concentration of the to-be-detected hormone is determined.

Description

technical field [0001] The invention relates to the field of medical equipment, in particular to a method and device for detecting low-abundance hormones. Background technique [0002] Hormones are roughly divided into four categories according to their chemical structures. The first category is steroids, the second category is amino acid derivatives, the third category hormones are peptides and proteins, and the fourth category is fatty acid derivatives. The secretion of hormones is extremely small, at the level of nanograms (that is, one billionth of a gram). The detection of target hormones takes a long time, requires a large amount of samples, and the price of detection equipment is high, and requires special professional skills, which is not suitable for routine clinical hormone monitoring. [0003] Taking estrogen belonging to the first category as an example, the content of estrogen in human blood is extremely low, at the pg / ml level, and the basic estrogen level in ...

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Problems solved by technology

In 2001, a multi-center, randomized double-blind controlled study promoted by the National Institutes of Health of the United States aimed at understanding the impact of hormone replacement on cardiovascular disease was terminated early due to adverse reactions, which greatly reduced the number of people receiving hormone replacement therapy. The suspicion of the increased incidence of breast cancer has made people afraid to use it lightly

Fully automatic immunobiochemical detectors have the characteristics of high sensitivity and high throughput, but the detection time is long, the sample volume is large (3-4ml of venous blood is required), and most of the immune analyzers are large-scale instruments, the price is high, and they are only suitable for The clinical laboratory or central laboratory of the hospital cannot realize instant detection and dynamic observation; liquid chromatography-tandem mass spectrometry, as the gold standard, has high precision and can detect estrogen with a lower limit of 5pg / ml, but the liquid separation steps are complicated. The equipment is expensive, requires special expertise, and is not suitable for routine clinical hormone monitoring

Therefore, for patients who need to dynamically monitor serum estrogen concentration, the existing detection methods will not only increase the economic burden and time loss of repeated visits to the doctor, but also cause multiple injuries to the body after repeated venous blood collection. At the same time, as long as 6-12 Hours of waiting for test results also bring a huge load to the outpatient department of the hospital

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