Culture method of selenium-rich high-cordycepin Cordyceps sinensis mycelia

A technology of cordycepin mycelium and high cordycepin, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of low content of cordycepin and low content of selenium, and achieve high content of selenium element , high labor efficiency and advanced methods

Inactive Publication Date: 2016-12-21
LUDONG UNIVERSITY
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Problems solved by technology

[0002] The existing culture methods of Cordyceps mycelium generally have problems such as low content of cordycepin and low content of selenium, which need to be further improved

Method used

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Embodiment Construction

[0033] A method for cultivating Cordyceps mycelium rich in selenium and high in cordycepin content, comprising the following steps:

[0034] (1) Activation of strains: Inoculate the strains of Cordyceps militaris in the following medium for activation and optimization: glucose 15-20 grams, magnesium sulfate 1-2 grams, potassium dihydrogen phosphate 1-2 grams, vitamin B10.1 -0.5 mg, 45-55 grams of white sea cucumber grinding liquid, 3-5 grams of yeast extract powder, 15-20 grams of agar powder, add water to 1000 ml; sterilize the above nutrient solution at 121 ° C, 0.1 MPa for 30 minutes, After cooling down, pour it into a petri dish; put the Cordyceps militaris strains into the petri dish, and cultivate it in the dark at 22°C for 5-10 days, select colonies with fast growth, dense hyphae, and pure color, and then transfer them to the slope Cultivate on the medium for 5-10 days in the dark at 22°C, and the resulting strain is the desired Cordyceps strain;

[0035] (2) Preparati...

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Abstract

The invention discloses a culture method of selenium-rich high-cordycepin Cordyceps sinensis mycelia. The method comprises the following steps: activating strains, preparing liquid primary strains, preparing liquid secondary strains, carrying out small strain fermentation in a tank, carrying out production fermentation in a tank, concentrating, and carrying out powder spray drying to obtain finished strain powder. A white beche-de-mer grinding liquid is adopted as an additive for a Cordyceps militaris culture medium, Cordyceps militaris mycelia with high selenium and high cordycepin are cultivated by using a liquid fermentation technology, the selenium content can reach 98.5 [mu]g/g or above, and the cordycepin content can reach 3.15 mg/g or above.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps mycelium. Background technique [0002] The existing methods for cultivating Cordyceps mycelium generally have problems such as low content of cordycepin and low content of selenium, which need to be further improved. Contents of the invention [0003] The object of the present invention is to provide a method for cultivating Cordyceps mycelium rich in selenium and high in cordycepin content, which is simple and convenient, has high content of cordycepin and high content of selenium. [0004] Technical solution of the present invention is: [0005] A method for cultivating Cordyceps mycelium rich in selenium and high in content of cordycepin, characterized in that it comprises the following steps: [0006] (1) Activation of strains: Inoculate the strains of Cordyceps militaris in the following medium for activation and optimization: glucose 15-20 grams, magnesium sulfate 1-2 grams, potassi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 周美红程显好王仲礼
Owner LUDONG UNIVERSITY
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