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Kit and method for in vitro directional differentiation of human-derived iPS stem cells into myocardial cells

A technology of cardiomyocytes and kits, applied in the fields of stem cell biology and regenerative medicine, which can solve the problems of high prices, great differences in cell lines, unfavorable large-scale production and application, and achieve high safety effects

Active Publication Date: 2016-12-21
广东依浦赛生物科技有限公司
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Problems solved by technology

[0007] Although there are currently many methods for directional differentiation of iPS cells into cardiomyocytes, the differentiation efficiency of cardiomyocytes is low, the price is high, and the differences between cell lines are large, which is not conducive to large-scale production and application.

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  • Kit and method for in vitro directional differentiation of human-derived iPS stem cells into myocardial cells
  • Kit and method for in vitro directional differentiation of human-derived iPS stem cells into myocardial cells

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Embodiment Construction

[0038] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available items.

[0039] As used herein, the term "induced pluripotent stem cell", often abbreviated as iPS cell, refers to an adult that has been reprogrammed to enter an embryonic stem cell-like state by being forced to express factors important for maintaining the "sternness" of embryonic stem cells cell. Generally, iPS cells are artificially prepared from non-pluripotent cells (such as adult human cells, or terminally differentiated cells), such as fibroblasts, hematopoietic cells, muscle cells, neurons, epidermal cells, etc., by The non-pluripotent cells are introd...

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Abstract

The invention provides a kit and a method for in vitro directional differentiation of human-derived iPS stem cells into myocardial cells. By virtue of a nutrient solution provided by the kit disclosed by the invention and in accordance with the method disclosed by the invention, the efficient and directional differentiation of the iPS stem cells into the myocardial cells is promoted. The kit and the method disclosed by the invention are simple and reliable, stable and efficient and high in safety, and the differentiation proportion of the multipotential stem cells into the myocardial cells is significantly increased to be greater than 90%. Meanwhile, the kit and a culture medium disclosed by the invention are applicable to the large-scale production of high-quality myocardial cells, without subsequent screening and purifying steps; and the kit and the culture medium are directly applicable to heart development scientific researches, cell therapy of heart diseases, transplantation for heart injury and application demand of drug screening.

Description

technical field [0001] The invention relates to the fields of stem cell biology and regenerative medicine, in particular to a kit and method for directional differentiation of human iPS stem cells into cardiomyocytes in vitro. Background technique [0002] With the aging population in China, cardiovascular disease has become a category of diseases that pose a great threat to health. In my country, the mortality rate of cardiovascular disease ranks second, and about 3 million people die from cardiovascular disease every year, especially myocardial infarction caused by coronary heart disease will cause part of the myocardial ischemia and necrosis, leading to cardiac arrhythmia in patients Insufficient blood pumping seriously threatens the health and quality of life of the Chinese people. At present, only cardiomyocytes differentiated from ES cells (embryonic stem cells) for patch replacement therapy is a feasible treatment method. However, the source of ES cell donor oocytes i...

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Application Information

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IPC IPC(8): C12N5/077
Inventor 潘淳刚池锦焕
Owner 广东依浦赛生物科技有限公司
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