Method for stimulation-induced rooting of crataegus cuneata test-tube plantlets using high concentration auxin

Abstract

The invention provides a method for stimulation-induced rooting of crataegus cuneata test-tube plantlets using high concentration auxin. The method comprises the specific steps that crataegus cuneata test-tube plantlets are selected form the sterile crataegus cuneata seedlings which are continuously subjected to multiplication culture of multiple shoots 5 times, and 2-3 pieces of base leaves of the test-tube plantlets are cut; then the crataegus cuneata test-tube plantlets of which the 2-3 pieces of the base leaves are cut are inoculated into 3 mg / L-5 mg / L of IBA auxin solution for stimulation, and the test-tube plantlets are inoculated in a blank culture medium after the stimulation is performed for 1-3 days, then the crataegus cuneata test-tube plantlets are treated in the IBA auxin solution for 1 day, thus the induced rooting culture of the crataegus cuneata test-tube plantlets is completed; the induced rooting seedlings of the crataegus cuneata are transplanted to a mixed soil comprising peat soil and vermiculite, and then the stimulation-induced rooting of the crataegus cuneata test-tube plantlets using the high concentration auxin is completed. The number of the obtained primary roots reaches 4.13 on average, the surviving rate is counted and reaches 88.6% after the hardening-seedlings are transplanted for 3 weeks. Compared with the prior rooting technique, the average number of the obtained primary roots is increased by 1.02, and the transplanting survival rate is increased by 72.2%.

Description

technical field [0001] The invention relates to a plant induced rooting culture, in particular to the induced rooting culture of wild hawthorn plants. Background technique [0002] Wild Hawthorn, Rosaceae Wild Hawthorn is a deciduous woody plant, mainly distributed in the northern, central and southern provinces of China; North Korea, Siberia, Russia and other regions. In my country, the suitable planting areas include Jiangsu, Zhejiang, Yunnan, Sichuan, Henan, Anhui and Hubei. Wild hawthorn has high medicinal, edible and landscape uses. In the original induction rooting of wild hawthorn test-tube plantlets, when the test-tube plantlets were at a constant auxin concentration level for a long time, the effect of auxin on the rooting of wild hawthorn test-tube plantlets showed a downward trend, and auxin could also inhibit the normal growth of roots in the later stage of culture. Contents of the invention [0003] The invention adopts the method that high-concentration aux...

AI Technical Summary

Problems solved by technology

Experiments have shown that this method of inducing rooting can effectively solve the problem of difficult rooting of test-tube seedlings of wild hawthorn, and greatly improve the survival rate of hardened seedlings for transplanting.

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