Application of sophoraflavanone G in preparation of drugs for killing ectoparasitic ciliates on fishes

A technology of dihydroflavonoids and ciliates, which is applied in the directions of drug combination, anti-infective drugs, pharmaceutical formulations, etc., can solve the problems of high residue and high toxicity, and achieves the effects of easy availability, low price and remarkable effect.

Active Publication Date: 2017-01-04
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some chemical drugs, such as malachite green and mercury preparations, have good insecticidal effe

Method used

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  • Application of sophoraflavanone G in preparation of drugs for killing ectoparasitic ciliates on fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: The killing effect of Sophora genus dihydroflavone G drug on the infective larvae of the multi-seed melon worm

[0019] (1) Preparation of medicinal solution: Weigh 3.84mg of Sophora dihydroflavone G into a 1.5mL EP tube, dissolve it with 20μL of absolute ethanol, then use distilled water to make a 2560mg / L drug stock solution, and prepare it by gradient dilution method to the required test concentration and stored in a 4°C refrigerator for later use. Taking the absolute ethanol content (0.13%) in the drug solution with the highest concentration as the standard, a control solution containing 0.13% absolute ethanol was prepared.

[0020] (2) Take 100 μL of worm liquid containing approximately 600 infectious larvae in a 96-well plate, and add 100 μL of different concentrations of liquid medicine to make the final concentration of 4, 2, 1, 0.5, 0.25, 0.125, 0.06, 0.03 and 0mg / L, each drug concentration set 3 parallel. Under the 4 × objective lens, the total...

Embodiment 2

[0023] Embodiment 2: Sophora genus dihydroflavone G medicine is to the killing effect of the multi-spun melon worm adult

[0024] (1) The preparation of the medicinal solution is the same as in Example 1.

[0025] (2) Take 200 μL of worm liquid containing about 60 adults in a 24-well plate, and add 200 μL of different concentrations of liquid medicine, so that the final concentration is 32, 16, 8, 4, 2, 1, 0.5, 0.25 and 0 mg / L, set 3 parallels for each concentration. Under the 4× objective lens, the total death time (min) of adults at each concentration within 5 hours and the number of adults surviving at the 5th hour were counted, and the mortality rate and half effective concentration (EC) were calculated. 50 ). The experimental temperature was maintained at 23±0.5°C. After 6 hours, count the number of cysts formed by surviving adults at each concentration under the 4× objective lens, and after 16 hours or 17 hours, count the number of infectious larvae released at each ...

Embodiment 3

[0029] Embodiment 3: Sophora genus dihydroflavonoid G is to the killing action of multi-seed melon worm cyst

[0030] (1) The preparation of the medicinal solution is the same as in Example 1.

[0031] (2) Add 200 μL of worm solution containing about 60 adults into a 24-well plate, place it in a constant temperature incubator at 23±0.5°C for 6 hours, and count the number of cysts formed in each well under a 4× objective lens. Subsequently, 200 μL of different concentrations of drug solutions were added to make the final concentrations 32, 16, 8, 4, 2, 1, 0.5, 0.25 and 0 mg / L, and three parallels were set for each concentration. Then the 24-well plate was placed in a constant temperature incubator at 23±0.5°C for 22 hours, the number of infectious larvae released at each concentration was counted under a 4× objective lens, and the average number of infectious larvae released per cyst at each concentration was calculated. Calculated as follows:

[0032] The average number of i...

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Abstract

The invention discloses application of sophoraflavanone G in the preparation of drugs for killing ectoparasitic ciliates on fishes and belongs to the technical field of drugs for killing ectoparasitic ciliates on fishes. Sophoraflavanone G is applied for the first time in the preparation of drugs for killing ectoparasites on fishes, particularly for killing Ichthyophthirius multifiis, the ectoparasites are completely killed in an effective dosage range, Ichthyophthiriasis is controlled, and significant control is provided for Ichthyophthiriasis; rich plants contain sophoraflavanone G and are low in price and easy to obtain, no residue or pollution is left, the plants are nontoxic to human, and an extraction and preparation process of sophoraflavanone G is simple and has a promising application prospect. Sophoraflavanone G is has median lethal concentration (46.6 mg/L) for grass carp that is 23.3 times the lowest effective concentration (2m g/L) to control Ichthyophthiriasis, is safe to fishes and has a promising application prospect.

Description

technical field [0001] The invention belongs to the technical field of drugs for killing fish parasites, and relates to a new application of dihydroflavone G of Sophora genus, in particular to an application of dihydroflavone G of Sophora genus in preparing a drug for killing ectoparasitic ciliates of fish. Background technique [0002] Parasitic ciliates are one of the most serious parasitic diseases that endanger the aquaculture industry. The multiseed melon worm is a worldwide distribution of ciliates that parasitize the body of fish. It mainly parasitizes the skin, gills and fins of fish, causing small melon worm disease, also known as "white spot disease", and causing serious damage to the aquaculture industry. Huge economic loss. Although some chemical drugs, such as malachite green and mercury preparations, have good insecticidal effects, they are highly toxic, have high residues, and have "three effects" effects, and their use has been banned. Therefore, there is a...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P33/14
CPCA61K31/352
Inventor 张其中林德杰
Owner JINAN UNIVERSITY
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