Application of Prunus persica bark extract in prevention and treatment of ichthyophthirius multifiliis disease of freshwater fishes

A technology of multi-seed melon worm and extract, which is applied in the field of fishery disease prevention and control, can solve the problems of lack of drug efficacy research, lack of peach bark, etc., and achieve the effect of abundant resources, easy acquisition, and simple and convenient extraction process

Pending Publication Date: 2021-10-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Peach tree (Prunus persica) is a traditional Chinese herbal medicine in my country, but its efficacy research is relative

Method used

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  • Application of Prunus persica bark extract in prevention and treatment of ichthyophthirius multifiliis disease of freshwater fishes
  • Application of Prunus persica bark extract in prevention and treatment of ichthyophthirius multifiliis disease of freshwater fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: The killing effect of the peach bark extract on the predators of the multiseed melon worm.

[0026] Pipette 100 μL of the predatory body liquid of the multi-spawned melon worm, about 60 worms, into a 96-well plate, a total of 5 experimental groups and 2 control groups, each group set up 3 parallels, a total of 2100 uL worm liquid. Dilute the drug mother solution to obtain the peach bark extract with a concentration gradient of 12.5, 6.2, 3.1, 1.6 and 0.8 mg / L, and add equal volumes (100 μL) to each experimental group so that the final concentrations of each group are 6.2, 3.1, 1.6, 0.8 and 0.4mg / L. 100 μL of 0.125% absolute ethanol was added to the ethanol control group, and 100 μL of aerated water was added to the blank control group. The 96-well plate was cultured in an incubator at 25°C. After 4 hours, the mortality of predators in each group was counted, and the status of the worms was observed under a microscope.

[0027] The results show that: when th...

Embodiment 2

[0028] Example 2: The killing effect of the peach bark extract on the trophozoites of the multiseed melon worm.

[0029] Draw about 30 worms in 1 mL of the trophozoite liquid of the multi-spawned melon worm into a 12-well plate, a total of 6 experimental groups and 2 control groups, and set up 3 parallels in each group, with a total of 24 mL of worm liquid. Dilute the mother liquor of the drug to obtain the ethanol extract of peach bark with a concentration gradient of 50, 25, 12.5, 6.25, 3.1 and 1.5mg / L, add equal volumes (1mL) to each experimental group, so that the final concentrations of each group are 25, 12.5 , 6.25, 3.1, 1.5 and 0.8mg / L. Add 1 mL of 0.5% absolute ethanol to the ethanol control group, and add 1 mL of aeration water to the blank control group. The 12-well plate was cultured in an incubator at 25°C. After 6 hours, the trophozoite mortality of each group was counted, and the state of the worm was observed under a microscope.

[0030] The results show that...

Embodiment 3

[0031] Example 3: The killing effect of the peach bark extract on the cysts of the multiseed melon worm.

[0032] Draw about 30 worms in 1 mL of the trophozoite liquid of the multi-spawned melon worm into a 12-well plate, a total of 6 experimental groups and 2 control groups, and set up 3 parallels in each group, with a total of 24 mL of worm liquid. Place the 12-well plate at 25°C and continue to incubate for 6 hours until the trophozoites in each well enter the encapsulation stage. Dilute the mother liquor of the drug to obtain the ethanol extract of peach bark with a concentration gradient of 200, 100, 50, 25, 12.5 and 6.25 mg / L, and add equal volumes (1 mL) into each experimental group so that the final concentrations of each group are 100, 50 mg / L, respectively. , 25, 12.5, 6.25 and 3.1mg / L. Add 1 mL of 2% absolute ethanol to the ethanol control group, and add 1 mL of aeration water to the blank control group. The 12-well plate was cultured in an incubator at 25°C. Afte...

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Abstract

The invention discloses application of a Prunus persica bark extract in prevention and treatment of the ichthyophthirius multifiliis disease of freshwater fishes, and belongs to the technical field of fishery disease prevention and treatment. In-vitro insecticidal test results show that the Prunus persica bark extract has a good killing effect for the grazing bodies, the trophozoites and the cysts of ichthyophthirius multifilis and has a dose-effect relationship so as to prove that the Prunus persica bark extract can be used for preventing and treating the ichthyophthirius multifiliis disease of the freshwater fishes by killing the ichthyophthirius multifilis body. The the ichthyophthirius multifiliis disease of freshwater fishes is used as a traditional Chinese medicine in China, and the extract of the peach bark has the characteristics of being safe and non-toxic to a water body environment and aquatic organisms, and has a huge application prospect in the field of novel green fishery medicines.

Description

technical field [0001] The invention belongs to the technical field of fishery disease prevention and control, and in particular relates to the application of a peach tree (Prunus persica) bark extract in the prevention and treatment of freshwater fish with many eggs. Background technique [0002] The multi-seed melon worm is a ciliated protozoan, which is widely parasitic on the body surface and gills of various freshwater fish, and can form white spots visible to the naked eye, and the disease caused is commonly known as white spot disease. After the fish is infected with the small melon worm, the diseased fish will be restless, loss of appetite, tissue hyperplasia, necrosis and other pathological symptoms in the parasitic site. When the gills are severely infected, the respiratory function of the fish will be impaired and eventually die. At present, chemical drugs such as formalin and copper sulfate are mainly used to prevent and control multi-seeded melon worms in aquacu...

Claims

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Application Information

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IPC IPC(8): A61K36/736A61P33/02A61K129/00
CPCA61K36/736A61P33/02A61K2236/333A61K2236/51
Inventor 顾泽茂杨浩胡静波肖杰尹李丹
Owner HUAZHONG AGRI UNIV
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