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Glycopeptide extract from ganoderma spore powder, method for preparing glycopeptide extract and application thereof

A technology of Ganoderma lucidum spore powder and spore powder sugar, which is applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of patients being unbearable, short survival time, and difficult to be widely used, and achieve low production cost, complete decolorization, and extraction good effect

Active Publication Date: 2017-01-04
BEIJING UNION PHARMA FACTORY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Infusion of white blood cells, on the one hand, is expensive and unaffordable for patients; on the other hand, the survival time of patients due to rejection activities is short, and it may promote the recurrence and metastasis of cancer
At present, colony cell stimulating factor (G-CSF) has been widely used to prevent and treat leukopenia caused by chemotherapy drugs, and the curative effect is better; patients' autologous bone marrow transplantation can also be used, but these treatments have higher medical and economic conditions. Requirements, it is difficult to be widely used clinically

Method used

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  • Glycopeptide extract from ganoderma spore powder, method for preparing glycopeptide extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: Extraction method one of Ganoderma lucidum spore powder glycopeptide

[0057] Put 1 kg of Ganoderma lucidum spore powder passed through a 80-mesh sieve into a round-bottomed flask, add 5 times the amount of 95% ethanol, heat at 80°C, and reflux for the first time of extraction. The extraction time is 2 hours, and suction filtration is obtained to obtain extract 1. Add 4 times the amount of 95% ethanol, heat at 80°C, reflux for the second and third times, extract for 1 hour, and filter with suction to obtain extracts 2 and 3, combine extracts 1, 2, and 3, and concentrate under reduced pressure to nothing Alcoholic taste, thick alcohol extract concentrate.

[0058] Continue to add 4 times the amount of 60% ethanol to the ganoderma lucidum spore powder dregs, heat at 90° C., and reflux extraction for 3 times, each time for 1 hour. Suction filtration to obtain extracts 4, 5, and 6, combined extracts 4, 5, and 6, concentrated under reduced pressure to obtain 3...

Embodiment 2

[0063] Embodiment 2: Extraction method two of Ganoderma lucidum spore powder glycopeptide

[0064] Put 1 kg of Ganoderma lucidum spore powder passed through a 80-mesh sieve into a round-bottomed flask, add 6 times the amount of 95% ethanol, heat at 80°C, and reflux for the first time of extraction. Add 5 times the amount of 95% ethanol, heat at 80°C, reflux for the second and third times, the extraction time is 1.5 hours, filter with suction to obtain extracts 2 and 3, combine extracts 1, 2 and 3, and concentrate under reduced pressure to nothing Alcoholic taste, thick alcohol extract concentrate.

[0065] Continue to add 5 times the amount of 60% ethanol to the ganoderma lucidum spore powder dregs, heat at 90° C., and reflux extraction for 3 times, each time for 1.5 hours. Suction filtration to obtain extracts 4, 5, and 6, combined extracts 4, 5, and 6, concentrated under reduced pressure to obtain 300ml of medicinal solution, added 8 times the amount (2.4L) of 95% ethanol f...

Embodiment 3

[0070] Example 3: Molecular weight detection of Ganoderma lucidum spore powder glycopeptide extract

[0071] The prepared Ganoderma lucidum spore powder glycopeptide was used to determine the molecular weight by high performance gel chromatography:

[0072] Instrument: Agilent1260 high performance liquid chromatography

[0073] Column:

[0074] Pre-column: TOSOH TSK-GUARDCOLUMN PW XL 6.0*40mm

[0075] TOSOH TSK-GEL G5000PW XL 7.8*300mm

[0076] TOSOH TSK-GEL G3000PW XL 7.8*300mm

[0077] Pre-column, G5000PW XL 、G3000PW XL in series

[0078] Mobile phase: 0.2mol / L sodium chloride aqueous solution

[0079] Flow rate: 0.6ml / min

[0080] Column temperature: 40°C

[0081] Detector: Agilent differential refractive index detector 40°C

[0082] Chromatography workstation: Agilent chromatography workstation (with GPC software)

[0083] Dextran standard molecular weight polysaccharide: molecular weight range 180-2000000, purchased from China Institute for the Control of P...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and relates to glycopeptide extract from ganoderma spore powder, a method for extracting the glycopeptide extract and application thereof. The method sequentially includes steps of extracting the ganoderma spore powder by the aid of concentrated alcohol; extracting the ganoderma spore powder by the aid of diluted alcohol; combining concentrated-alcohol extract liquid and diluted-alcohol extract liquid with each other to obtain mixed extract liquid and degreasing the mixed extract liquid by the aid of diethyl ether; removing impurities from the mixed extract liquid and decolorizing the mixed extract liquid; purifying the mixed extract liquid by the aid of resin. The method preferably further includes steps of further treating ganoderma spore powder residues by the aid of aqueous extraction, alcohol precipitation and refining steps. The ganoderma spore powder residues remain after the ganoderma spore powder is extracted by the aid of the concentrated alcohol and the diluted alcohol. The glycopeptide extract is prepared by the aid of the method. The glycopeptide extract, the method and the application have the advantages that the method for extracting the glycopeptide extract from the ganoderma spore powder is easy and convenient to implement and low in cost and is environmentally friendly and safe, good extraction effects can be realized, structures of the glycopeptide extract can be prevented from being destroyed, solvents can be recycled, and industrial production can be facilitated; excellent effects can be realized by the glycopeptide extract when the glycopeptide extract is used for increasing leukocytes.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a ganoderma lucidum spore powder glycopeptide extract and a preparation method and application thereof. Background technique [0002] Leukopenia (abbreviation leucopenia) is a group of syndromes caused by a variety of etiologies. Leukopenia is defined as the total number of peripheral white blood cells continuously lower than 4.0×10 9 L / L is the main diagnostic basis, and it is a common disease in clinical medicine at present. According to its etiology, it can be divided into two types: unexplained and secondary. The leukopenia secondary to tumor chemotherapy and radiotherapy is different from that secondary to other infectious diseases (such as typhoid, paratyphoid, yellow fever, etc.), and the occurrence of this serious side effect often makes tumor radiotherapy and chemotherapy difficult. forced to stop. In recent years, many medical institutions in China have give...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61P7/00
Inventor 何秀峰夏鹏飞雷鸣代春红赵立敏
Owner BEIJING UNION PHARMA FACTORY
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