Live vaccine composition containing adjuvant and preparation method and application of live vaccine composition
A vaccine composition and composition technology, applied in the direction of medical preparations containing active ingredients, antiviral agents, pharmaceutical formulations, etc., can solve the restrictions on the application and promotion of live vaccines, the absence of adjuvants, and the reduced ability of immune response And other issues
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Embodiment 1
[0045] The preparation of embodiment 1 adjuvant
[0046] Disperse Carbomer 934P (Qingdao Tianliyuan Biotechnology Co., Ltd.), chitosan (Shanghai Qisheng Biological Products Co., Ltd.), and Pronick L121 (Caisson Labs, USA) into purified water to prepare a stock solution, 12°C for 30min Autoclave and store at 4°C. The specific formula is shown in Table 1.
[0047] Table 1 Adjuvant formulation
[0048] Element Z1 Z2 Z3 Z4 Z5 Z6 Z7 Z8 Carbomer wt% 0.01 0.1 0.5 2 0.01 0.1 0.5 2 Chitosan wt% 0.1 0.5 1 5 0.1 0.5 1 5 Pronic L121wt% 0.5 1 4 5 0 0 0 0
[0049] Adjuvants Z1 and Z5 were prepared into solutions Z11 and Z51 with a particle size of 0.01 μm, Z2 and Z6 were prepared into solutions Z21 and Z61 with a particle size of 0.1 μm, and Z3 and Z7 were prepared into solutions Z31 and Z31 with a particle size of 2 μm. Z71, Z4, Z8 were prepared into solutions Z41, Z42, Z43 and Z81, Z82, Z83 with particle sizes of 5 μm, 6 μm, ...
Embodiment 2
[0050] Preparation of embodiment 2 porcine pseudorabies virus Bartha K-61 strain attenuated live vaccine composition
[0051] The adjuvant prepared in Example 1 was matched with the freeze-dried antigen of the porcine pseudorabies virus live vaccine BarthaK-61 strain (produced by Spain's Haibolai Biopharmaceutical Factory, batch 77BN) to form a live vaccine combination containing adjuvant When used, the adjuvant and the antigen can be mixed evenly, and the specific matching method is shown in Table 2.
[0052] Table 2 The ratio of the attenuated live vaccine composition of porcine pseudorabies virus Bartha K-61 strain
[0053]
Embodiment 3
[0054] Example 3 Porcine pseudorabies virus Bartha K-61 strain attenuated live vaccine composition immunogenicity test one
[0055] 65 9-day-old PRV antigen-antibody-negative piglets were randomly divided into 13 groups, 5 piglets / group, and immunized with the attenuated live vaccine of porcine pseudorabies virus Bartha K-61 strain prepared in Example 2. The first group of nasal drop immunization vaccine 1, the second group of intramuscular injection of vaccine 1, the third group of nasal drop immunization vaccine 2, the fourth group of intramuscular injection of vaccine 2, the fifth group of nasal drop immunization vaccine 3, the sixth group of intramuscular injection Immune vaccine 3, the seventh group of intranasal immunization vaccine 4, the eighth group of intramuscular injection of immunization vaccine 4, the ninth group of nasal immunization vaccine 5, the tenth group of intramuscular injection of immunization vaccine 5, the eleventh group of nasal immunization vaccine 6...
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