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Method for producing pullulan with different molecular weights by semi-continuous method

A pullulan polysaccharide and molecular weight technology, which is applied in the field of semi-continuous production of pullulan polysaccharides of different molecular weights, can solve the problems of different molecular weight requirements, polymerization degree changes, molecular weight differences of pullulan polysaccharides, and achieves high repetition rate and fermentation. Stable, short fermentation time effect

Inactive Publication Date: 2017-01-04
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different properties of pullulan required in different fields, resulting in differences in the molecular weight requirements of pullulan
Pullulan polysaccharides are subject to changes in the degree of polymerization due to differences in bacterial species and fermentation conditions, which affects the difference in molecular weight

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] After activating Aureobsidium pullulans (Aureobsidium pullulans) CGMCC NO.7055 strain for 6~8 hours, transfer it from the PDA slant medium to 500mL seed medium with a liquid volume of 100mL. The mass ratio of the seed medium is as follows : 100 parts of sucrose, 3 parts of yeast extract powder, 2 parts of K2HPO4, 2.5 parts of NaCl, 0.4 parts of MgSO4, 1 part of (NH4)2SO4, 0.05 parts of FeSO4, pH is 6.0, the seed liquid is placed in the above medium at 28 ° C, Cultivate at 180rpm for 32h to obtain the fermented seed liquid, then transfer 3% inoculum into a 5L fermenter with a liquid volume of 3L, the ventilation rate is 1.25V / V, the stirring speed is 400rpm, and the cultivation temperature for the first 24h is 32°C After 24 hours, the fermentation temperature was adjusted to 28°C.

[0020] Release 40% of the fermentation broth after 48 hours of fermentation, which is recorded as "the first batch", and then supplement the fermentation medium with the same discharge volume...

Embodiment 2

[0023] After activating Aureobsidium pullulans (Aureobsidium pullulans) CGMCC NO.7055 strain for 6-8 hours, transfer it from PDA slant medium to 500mL medium with a liquid volume of 100mL, and cultivate the seed solution at 28°C and 180rpm for 32h. Then 5% of the inoculum was inserted into a 5L fermenter with a liquid volume of 3L, the ventilation rate was 1.0V / V, the stirring speed was 400rpm, the incubation temperature was 32°C for the first 24h, and the fermentation temperature was adjusted to 28°C after 24h.

[0024] After the fermentation was carried out for 56 hours, 45% of the fermentation liquid was released, which was recorded as "the first batch", and then the same volume of fermentation medium was added to it, and the pH was adjusted to 4.0. Continue to ferment for 56h and then emit 60% of the fermented liquid, and this batch of fermented liquid is denoted as "the second batch". Then continue to supplement the same fermentation medium, adjust the pH to 3.5, stop the...

Embodiment 3

[0027] After activating Aureobsidium pullulans (Aureobsidium pullulans) CGMCC NO.7055 strain for 6-8 hours, transfer it from PDA slant medium to 500mL medium with a liquid volume of 100mL, and cultivate the seed solution at 28°C and 180rpm for 28h. Introduce it into the secondary seed solution for 24 hours at 28°C and 200rpm. Put 5% of the inoculum into a 30L fermenter with a liquid volume of 18L, the ventilation rate is 1.15V / V, the stirring speed is 400rpm, the cultivation temperature is 32°C for the first 24h, and the fermentation temperature is adjusted to 28°C after 24h.

[0028] After 48 hours of fermentation, 45% of the fermentation broth was released, which was recorded as "the first batch", and then the same volume of fermentation medium was added to it, and the pH was adjusted to 3.5. Continue to ferment for 60h and then emit 60% of the fermented liquid, and this batch of fermented liquid is denoted as "the second batch". Then continue to supplement the same ferment...

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Abstract

The invention discloses a method for producing pullulan with different molecular weights by a semi-continuous method. The method is characterized by comprising the following steps of inoculating a fermented seed solution into a fermentation medium; draining a certain amount of fermentation broth in the fermenting process by adopting semi-continuous fermentation; supplementing a fresh fermentation medium with the same volume, adjusting a pH value, and continuously fermenting; after fermentation for a certain time, continuously draining the fermentation broth, and supplementing the fresh fermentation medium with the same volume; then adjusting a pH value, repeatedly operating, and adjusting pH values of fermentation to be different, thus obtaining the fermentation broth containing the pullulan with the different molecular weights. According to the method disclosed by the invention, an effect of obtaining the pullulan with the different molecular weights is realized by combining the semi-continuous fermentation with adjustment of different pH values of the fermentation. Compared with a method of obtaining the pullulan with the different molecular weights by batch fermentation in batches, the method disclosed by the invention has the advantages that fermentation time required by the semi-continuous fermentation is shorter, and the repetitive rate of obtaining the pullulan with the same molecular weight is higher; in addition, by adopting the mode, the fermentation is more stable.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and relates to a semi-continuous method for producing pullulan with different molecular weights. Background technique [0002] Pullulan has good physical and chemical properties, so it has the potential to be used in many fields such as food, medicine, chemical industry, environmental protection, and packaging. Due to the different properties of pullulan required in different fields, there are differences in the molecular weight requirements of pullulan. The degree of polymerization of pullulan is affected by different strains and fermentation conditions, which affects the difference in its molecular weight. It is reported that the weight average molecular weight of pullulan can be controlled at 2.8×105~6.0×105, and the molecular weight dispersion index is 2.0~2.4 by using different organic nitrogen sources. In addition, pullulan with different molecular weights can be obtained by control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/10C12R1/645
CPCC12P19/10
Inventor 乔长晟王萌李雪
Owner 天津北洋百川生物技术有限公司
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