A method of improving coalbed methane production by using native bacteria
A technology of coalbed methane and native bacteria, which is applied in the preparation of test samples, bacteria, and earth-moving drilling, etc., can solve the problems of high energy consumption, long processing time, low work efficiency, etc., to improve coal seam structure and reduce energy consumption and enhance efficiency
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Embodiment 1
[0039] A kind of method that utilizes native bacterium to improve coalbed methane output provided by the invention, the steps are as follows:
[0040] (1) The target coalbed methane field is a certain area in the south of the Junggar Basin in Xinjiang. Coal samples from coalbed methane reservoirs are collected, and samples are taken at the wellhead of the coalbed methane. The sample bottles are pre-sterilized and filled with nitrogen. The air remains, and it is sent into the anaerobic incubator in a shading manner within 24 hours; the colony structure is extracted from the coal sample and water sample, and the microbial community structure of the coal sample and water sample is analyzed by cloning library technology For analysis, the bacterial species were quantitatively analyzed by qPCR technology.
[0041] The target coal seam for CBM development is the coal seam of the Xishanyao Formation in the Middle Jurassic, with a reservoir pressure of 3.8MPa, a reservoir temperature o...
Embodiment 2
[0074] On the basis of the foregoing embodiment, the optimal parameters obtained in the step (5) are: the optimal parameter pressure of the ultrasonic treatment is 0.8Mpa, the ultrasonic frequency is 80kHz, the processing time is 20min, and the supercritical CO 2The optimal parameter pressure for processing is 8.2MPa, and the processing time is 30h. Under this condition, the ultrasonic coupling supercritical CO 2 The amount of methane produced by biodegradation per unit mass of coal after treatment is the largest.
[0075] The gas composition and methane concentration change at the gas production port in the step (9) are measured by a methane analyzer and a gas chromatograph.
Embodiment 3
[0077] On the basis of the above-mentioned embodiments, in the step (2), each bacterial strain combination is added with anaerobic fermentation bacteria to enhance the degradation degree of coal, and determine the most favorable bacterial colony combination for gas production in the target coal seam through the collocation of different combinations, and at the same time Avoid inhibition between colonies.
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