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Method for measuring content of methoxyl in pectin

A methoxyl content technology, applied in the field of analysis and detection, can solve problems such as poor repeatability and unstable measurement results, and achieve the effects of reduced measurement errors, short measurement process time, and accurate measurement results

Active Publication Date: 2017-01-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Colorimetric method is rarely used now because of the need to add special reagents and the measurement results are unstable and poor in repeatability

Method used

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  • Method for measuring content of methoxyl in pectin
  • Method for measuring content of methoxyl in pectin
  • Method for measuring content of methoxyl in pectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 measures the content of methoxyl group in high methoxyl pectin

[0028] (1) Preparation of methanol standard solution: accurately measure 0.800 g of methanol (gas chromatography standard product) in a volumetric flask, add distilled water to make the volume to 1000 mL to obtain methanol standard solution;

[0029] (2) Establishment of standard curve: add 0, 0.005, 0.01, 0.015, 0.02, 0.03, 0.04mL methanol standard solution to different sealed headspace sample vials in turn, and place the prepared headspace sample vials in On the sample stage, then perform headspace-gas chromatography detection, wherein the operating conditions of the headspace sampler are: heating furnace temperature 110 ° C; the equilibrium time of the headspace bottle in the oven is 5.0min, and the gas chromatography operating conditions are: column temperature The temperature is 35°C; the carrier gas is helium, the flow rate is 6.0mL / min, and the detector temperature is 250°C. Then obtai...

Embodiment 2

[0035] Embodiment 2 measures the content of methoxyl group in low methoxyl pectin

[0036] (1) Preparation of methanol standard solution: accurately measure 0.200 g of methanol (gas chromatography standard product) in a volumetric flask, add distilled water to make the volume to 1000 mL to obtain methanol standard solution;

[0037] (2) Establishment of standard curve: add 0, 0.005, 0.01, 0.015, 0.02, 0.03, 0.04mL methanol standard solution to different sealed headspace sample vials in turn, and place the prepared headspace sample vials in On the sample stage, then carry out headspace-gas chromatography detection, the operating conditions of the headspace sampler are: heating furnace temperature 110 ℃; the equilibrium time of the headspace bottle in the oven is 5.0min, the gas chromatography operating conditions are: 35°C; the carrier gas is helium, the flow rate is 6.0mL / min, and the detector temperature is 250°C. Then obtain a calibration curve according to the correspondin...

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Abstract

The invention belongs to the field of analysis detection, and particularly relates to a method for measuring content of methoxyl in pectin by using a phase inversion method in combination with a headspace gas chromatography technology. The method comprises the following steps: (1) preparation of a standard solution; (2) establishment of a standard curve; (3) pretreatment of a sample; (4) analysis by using a headspace gas chromatography; (5) calculation of results. The adopted method for measuring content of methoxyl in pectin has advantages of accurate measurement results, simple and rapid operation, high detection sensitivity and the like.

Description

technical field [0001] The invention relates to a method for measuring the methoxyl content in pectin, in particular to a method for measuring the methoxyl content in pectin by using phase inversion technology combined with headspace gas chromatography, and belongs to the field of analysis and detection. Background technique [0002] Pectin is a natural additive extracted from plant fruits. It is a polymer compound with galacturonic acid as the basic unit, and its molecular weight is about 1,000 to 40,000. Pectin exists in all green plant tissues, has good gelation and emulsification stabilization, and is widely used in food industry, cosmetics and medicine. As a food gelling agent, emulsifier and stabilizer, pectin mainly depends on the content of polygalacturonic acid and methoxyl in the molecular structure. The absolute theoretical value of methoxy in pectin is 16.3%. Therefore, the content of methoxyl group is also an extremely important parameter to measure the proper...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李海龙杜超刘梦茹
Owner SOUTH CHINA UNIV OF TECH