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Chiral analysis method for nicotine in tealeaves

An analysis method and technology of nicotine, which is applied in the field of chiral analysis of nicotine in tea, to achieve the effect of simple and fast operation, high sensitivity and strong anti-interference ability

Active Publication Date: 2017-01-04
CHINA TOBACCO GUANGXI IND
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of nicotine in tea is much lower than that in tobacco, which brings challenges to the chiral analysis of nicotine in tea

Method used

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  • Chiral analysis method for nicotine in tealeaves
  • Chiral analysis method for nicotine in tealeaves
  • Chiral analysis method for nicotine in tealeaves

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0037] The present invention is further described by the following specific examples, but does not limit the present invention.

[0038] 1. Instruments and Reagents

[0039] MDGCMS-QP2010Ultra multidimensional gas chromatography spectrometer (Shimadzu Corporation of Japan), automatic sampler AOC-5000 (Shimadzu Corporation of Japan), automatic solid phase microextraction fiber injection needle, 65 μm PDMS / DVB (polydimethylsiloxane Alkane / divinylbenzene) stationary phase extraction fiber head (Supelco, USA), Rtx-5MS chromatographic column (30m×0.25mm×0.25μm, Shimadzu Corporation, Japan), InertCap CHIRAMIX chromatographic column (30m×0.25mm×0.25μm, Japan Shimadzu Corporation), AE163 electronic balance (sensitivity: 0.0001g, Swiss Mettler Company), HY-8 speed-adjustable oscillator (Changzhou Guohua Electric Co., Ltd.), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), Germany SIGMA 3-30K-high-speed table-top refrigerated centrifuge. n-Hexane (chromatographically pure), ...

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Abstract

The invention discloses a chiral analysis method for nicotine in tealeaves. The chiral analysis method is characterized by comprising the following steps: drying, crushing and sieving a tealeaf sample, adding extraction liquid for extraction and filtration, performing multidimensional gas chromatography-tandem mass spectrometry analysis after automatic solid-phase micro-extraction, and quantitatively detecting the proportion of S-(-)-nicotine and R-(+)-nicotine in the tealeaves by adopting a peak area normalization method. According to the analysis method, the sample can be simply and rapidly treated, the method is low in detection limit and high in sensitivity and accuracy, and the chiral analysis requirement of the nicotine in the tealeaf sample can be met.

Description

technical field [0001] The invention relates to a method for determining chemical components of tea, in particular to a chiral analysis method for nicotine in tea. Background technique [0002] Nicotine, also called nicotine, is an alkaloid present in Solanaceae plants and an important component of tobacco. Studies have shown that tea also contains nicotine, and its content is about 163-1600μg / kg. The nicotine molecule contains a chiral center, which is the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. The physiological activity and toxicity of S-(-)-nicotine are higher than that of R-(+)-nicotine. The affinity of nicotine is 10-100 times higher. Therefore, the chiral analysis of nicotine in tea is of great significance to understand the health risks of nicotine ingestion through ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/68G01N30/78
CPCG01N30/06G01N30/68G01N30/78G01N30/88G01N2030/062G01N2030/685G01N2030/884
Inventor 李小兰周芸刘鸿黄善松林莉许蔼飞蒋宏霖蒋光辉周奕潘玉灵
Owner CHINA TOBACCO GUANGXI IND
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