Method for separating and purifying eurotium cristatum from fu-brick tea and liquid culture method of eurotium cristatum

Abstract

The invention discloses a method for separating and purifying eurotium cristatumm from fu-brick tea and a liquid culture method of the eurotium cristatum. The liquid culture method includes the steps of 1), preparing a flat solid culture medium; 2), performing inoculation; 3), placing the flat solid culture medium after inoculation in an incubator for culture; 4), transferring to the flat solid culture medium newly prepared in the step 1); 5), repeating the step 3) and the step 4) twice to four times till pure bacterial colony of the eurotium cristatum is obtained to obtain the purified eurotium cristatum; 8), placing strains obtained in the step 7) in an incubator for culture with the temperature of 25-30DEG C for culture for 4-7 days when necessary. With the method, original region and environment growth restriction of the eurotium cristatum is broken through under human intervention, artificial mass culture regardless of area and environment is realized, technical support is provided for inoculating the eurotium cristatum on other tea or products for fermentation to produce products of other types, and the method is provided for purely cultivating the eurotium cristatum to obtain beneficial ingredients therein.

Description

technical field [0001] The invention belongs to the field of coronoid saccharomyces culture, and relates to a method for separating and purifying saccharomyces coronis from Fuzhuan tea and a liquid culture method thereof. Background technique [0002] In today's society, the economy is developing rapidly, and the industry that dominates economic development provides an environment for people to live in. As a fungus of the genus Trichomonas, it is also known as "Golden Flower" fungus, because it is mainly used in the fermentation process of Fucha It is named after a large number of golden closed capsules. [0003] For a long time, the production of Fuzhuan brick tea has mainly relied on natural fermentation to finally obtain the finished product. There are strict requirements on the production environment and origin. Therefore, the production of Fuzhuan brick tea is greatly restricted. Controlling the fermentation conditions can effectively break through the limitations of t...

AI Technical Summary

Problems solved by technology

[0004] The invention patent with the application number of 201010598201.8 provides a rapid separation method for the coronoid saccharomyces. First, the finished Fuzhuan brick tea is pulverized, and then it is made into a suspension and then coated on a flat plate. This method is not easy to operate, and the tea residue and crown Suspension prepared from the mixture of Closthesia cystocystis, which has a high risk of contaminating bacteria