A kind of preparation method of coix coix water-soluble cellulose with high purity and high yield

Abstract

The invention discloses a preparation method of a high purity and high yield Coix lacryma-jobi water-soluble cellulose. The method includes the steps of: (1) adding water that is 30 times the amount of a whole coix seed into the coix seed, performing heating to boiling and conducting extraction for 1h, and repeating extraction three times; (2) discarding the three aqueous extract solutions, adding water that is 30 times in amount into the residue, conducting heating boiling to a soft and mushy state, performing crumbing, conducting flushing under a small water flow, carrying out sieving by a 20-mesh sieve, and discarding hila; (3) adding a 30 times amount of water, performing heating to boiling and conducting extraction for 10h, and repeating extraction twice; (4) conducting standing for 12h, taking the supernatant, and carrying out concentration to a relative density of 1.30; (5) adding a 4 times volume of 95% ethanol, conducting full stirring, performing room temperature precipitation for 12h, and collecting the precipitate; and (6) conducting drying, and crushing the product to an appropriate particle size, thus obtaining the finished product Coix lacryma-jobi water-soluble cellulose. The method provided by the invention has the advantages of no toxicity, strong operability and high yield, etc., and is suitable for popularization.

Description

technical field [0001] 本发明涉及一种制备方法,为高纯度和高收率薏苡水溶性纤维素的制备方法。 Background technique [0002] 薏苡仁为禾本科植物薏苡的成熟种仁,味甘淡,性微寒,具有健脾渗湿,除痹止泻,清热排浓的功效。薏苡仁作为常用中药已有1500年的应用历史,目前被我国卫生部列为珍贵的药食两用的药物和食物,作为药食同源的作物原料,薏苡仁的医疗保健品质令人瞩目。薏苡仁含有多种成分,其中薏苡仁的主要活性成分为薏苡水溶性纤维素,具有调节免疫、抗氧化、抗衰老、降血脂、降血糖、抗肿瘤等作用。临床上薏苡仁可治疣平痘,淡斑美白、润肤除皱、美容养颜等。 [0003] 国内外对薏苡仁开发利用极为重视,薏苡水溶性纤维素的需求越来越多,对薏苡仁水溶性纤维素的提取和纯化技术要求也越来越高。目前国内文献报道的从薏苡仁中提取水溶性纤维素的主流方法是水提醇沉法,该方法提取阶段一般提取2-3次,而且由于担心加热提取会糊底,因此通常采用整粒提取。这种方法存在的主要问题是由于薏苡仁富含木质纤维的特性,整粒提取很难通过2-3次能够提取出来,相反由于薏苡仁的结构特性,前2-3次提取出来的大多为黑色色素、蛋白质,以及水溶性淀粉的混合物,而大部分真正的水溶性纤维素尚包裹于煮开花的薏苡仁中。特别是当种脐一直混存于被提取部分中,种脐中的脂溶性成分一直作为杂质,不断与薏苡水溶性纤维素一起被提取,混存于薏苡水溶性纤维素中。因此用目前的提取方法不仅收率很低,只有20%左右,而且薏苡水溶性纤维素的含量也很低,杂质较多,通常水溶性纤维素的含量仅有大约60%,致使后期需要采用复杂的酶解或有机溶剂萃取脱蛋白、氧化脱色,甚至采用色谱柱进行精制处理,方能提高薏苡水溶性纤维素的纯度。本发明提供了一种制备高纯度薏苡水溶性纤维素的方法,解决了现有技术中薏苡水溶性纤维素提取收率低,纯度不高,尚需后续采用有机溶媒脱蛋白,以及色谱柱纯化精制等复杂步骤的技术问题。另外由于本工艺的前三次提取已经同时去除了大部分可溶性淀粉,因此捏碎后的薏苡悬浮液并不存在提取糊底的现象。采用本发明工艺仅使用水醇处理方法,就能得到收率高达60%以上,水溶性纤维素含量高达95%-100%,色泽纯白优质薏苡水溶性纤维素。 Contents of the invention [0004] 本...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing high-purity coix coix water-soluble cellulose to solve the problem of low extraction yield and low purity of coix coix water-soluble cellulose in the prior art. Proteins, as well as the technical problems of complex steps such as purification and purification of macroporous adsorption resin chromatographic columns