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A rapid propagation method of Dendrobium candidum

A Dendrobium officinale, fast technology, applied in the field of rapid propagation of Dendrobium officinale, can solve the problems of preservation and rapid propagation of protocorms, and achieve good ecological effects, rapid and stable proliferation, and low cost effects

Inactive Publication Date: 2017-01-25
LIUZHOU HONGJI AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is easy to differentiate into protocorms in solid medium, and then develop into seedlings, but the purpose of preservation and rapid propagation of protocorms cannot be achieved

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0013] The rapid propagation method of Dendrobium candidum provided by the invention is described in detail as follows:

[0014] Step A, prepare an effective medium: the medium is prepared by using B5 basic medium, adding 0.6-0.9mg / L NAA, 0.3-0.6mg / L6-BA and 210-300g / L potato juice, and sterilizing at 135°C for 18 minutes; Among them, adding 0.8mg / L NAA, 0.5mg / L6-BA and 280g / L potato juice and sterilizing at 135℃ for 18min is the best solution.

[0015] Step B, transfer the protocorm to the prepared medium.

[0016] Step C, culture conditions: the light time is 9-11 hours per day, the light intensity is 2500 lx, and the temperature is 27°C. Among them, 10 hours of light time per day is the best solution.

[0017] The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be ...

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PUM

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Abstract

The present invention is applicable to the field of plant culture technology improvement, and provides a method for rapid propagation of Dendrobium candidum, which comprises the following steps:A, configuration of effective medium: The B5 basic medium is used for culturing, 0.6 - 0.9mg / L NAA, 0.3 - 0.6mg / L6 BA and 210 - 300g / L potato juice are added and sterilized at 135 oC for 18min; Transferring the original bulb to the configured medium;C, culture conditions: the light time is 9-11 hours a day, the light intensity is 2500lx, and the temperature is 27 oC.Using a unique liquid culture formula for the quick and stable proliferation of protocorms, providing a short cycle, high rate of reproduction, low cost of seedling methods for the large-scale cultivation of Dendrobium candidum.

Description

technical field [0001] The invention belongs to the field of biotechnology improvement, in particular to a rapid propagation method of dendrobium officinale. Background technique [0002] Dendrobium candidum is listed as "the first of the nine Chinese immortal grasses", and has been listed as a royal tribute since the Tang and Song Dynasties. Li Shizhen commented in "Compendium of Materia Medica" that Dendrobium candidum "strengthens yin and nourishes essence, thickens stomach, nourishes internal deficiency, calms stomach qi, grows muscles, improves intelligence and eliminates shock, lightens body and prolongs life"; folks call it "life-saving fairy grass" . In addition, the stems of Dendrobium candidum can clear away heat and promote body fluid, reduce inflammation and relieve pain, clear and moisten the throat, and have a good effect on the treatment of hoarseness. [0003] Dendrobium officinale generally grows at an altitude of 100-3000 meters above sea level, often epi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001
Inventor 黄茜菲
Owner LIUZHOU HONGJI AGRI TECH