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Application of ascaroside C9 in prevention and control of monochamus alternates and intercepting of spreading of pine wood nematode

A technology of monochamus alternatus and pine xylophilus, applied in the direction of application, nematicides, insecticides, etc., can solve the problems of inability to emerge, unable to form and cut off the spread, delayed emergence time of monochamus alternatus, and achieve Easy to popularize and apply, stable structure, obvious control effect

Inactive Publication Date: 2017-01-25
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, interfering with and regulating the development of Monochamus alternatus larvae can delay or prevent the eclosion of Monochamus alternatus, thus making L. Ⅳ Unable to form and cut off the way of its spread

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In the area where B. xylophilus occurs, before the pupation of Monochamus alternatus begins at the end of April and the beginning of May, 40 Pinus massoniana plants containing multiple monochamus alternatus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains were used as the experimental group, and 20 strains were used as the control group. On the sunny side of the tree body, the xylem monochamus alternata bored into the hole with an electric drill to drill a hole with a diameter of 1 cm and a depth of 2 cm, which communicated with the pupal chamber of the monochamus alternatus larvae. Add ascaroside C9 dosage 6nM / well directly to the wells of the experimental group, and the dosage of ascaroside C9 is 30nM / m 3 Pine tree body. Ascaroside C9 was not added to the control gro...

Embodiment 2

[0024] Ascaroside C9 was dissolved in methanol and adsorbed on a rubber septa (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 40 Pinus massoniana plants containing multiple monochamus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill in the xylem of the tree body on the sunny side of the tree. In the experimental group, a number of silicone rubber stoppers containing 1 μl of ascaroside C9 were scattered and inserted into the holes (after mixing 5 μl of ascaroside C9 and 100 μl of methanol, 20 μl was dropped into the silicone rubber stoppers, and used after the solvent evaporated). The d...

Embodiment 3

[0026] Ascaroside C9 was dissolved in ethanol and adsorbed on a silicone rubber plug (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 40 Pinus massoniana plants containing multiple monochamus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill in the xylem of the tree body on the sunny side of the tree. In the experimental group, a number of silicone rubber plugs containing ascaroside C9 were scattered and inserted into the holes (take 20 μl of 3 μM ascaroside C9 ethanol solution and drop it into the silicone rubber plug, and it can be used after the solvent evaporates). The dosage of asca...

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Abstract

The invention relates to application of ascaroside C9 in prevention and control of monochamus alternates and intercepting of spreading of pine wood nematode. The method for preventing and controlling the monochamus alternates by using the ascaroside C9 comprises the following step: placing the ascaroside C9 in a pine tree containing monochamus alternates larvae. The method provided by the invention can be used for treating forest monochamus alternates and pine trees infected with the pine wood nematode. The problems that the population quantity of the monochamus alternates in a pine wood nematode epidemic place is large, hazards are severe, and the pine wood nematode is diffused rapidly by the monochamus alternates can be solved.

Description

technical field [0001] The invention relates to the application of ascaroside C9 in the prevention and control of monochamus monochamus and the interception of the spread of pine xylophilus. Background technique [0002] Monochamus alternatus does not occur much in areas where pine wood nematode does not occur, and the damage is not enough to attract people's attention. After the pine wood nematode invaded China, it cooperated with the medium monochamus monochamus, and the population densities of both were greatly increased, forming a trend of rapid expansion and spread. [0003] Pine wood nematode disease, known as the "cancer" of pine trees, is the most dangerous forest disease in the world. It is a devastating disease of pinaceae plants. The host plant can die within 2-3 months after being infected with the disease, and 3- The entire pine forest can be destroyed within 5 years, which is extremely harmful. Since the first discovery of pine wood nematode disease in Nanjin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P5/00A01P7/04A01G13/00
CPCA01N43/16A01G13/00
Inventor 赵莉蔺孙江华
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI