Genetically engineered bacteria for producing compound carotenoids as well as construction method and application of genetically engineered bacteria

Abstract

The invention discloses genetically engineered bacteria for producing compound carotenoids (including [alpha]-carotene, [beta]-carotene, [delta]-carotene and lycopene) as well as a construction method and an application of the genetically engineered bacteria. The genetically engineered bacteria for producing the compound carotenoids are obtained by linking a musa nana luor. lycopene [epsilon]-cyclase gene to an expression vector and then transforming the gene linked with the expression vector into engineering bacteria containing LYC-LCYB1 plasmids, wherein the nucleotide sequence of the musa nana luor. lycopene [epsilon]-cyclase gene is shown as SEQ ID NO.1. With the application of the genetically engineered bacteria provided by the invention, the compound carotenoids, which are high in proportion of the [alpha]-carotene, can be directly obtained from the bacterium body; and the genetically engineered bacteria have a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a genetically engineered bacterium for producing compound carotenoids (α-carotene, β-carotene, δ-carotene and lycopene) and its construction method and application. Background technique [0002] Carotenoids can become an important provitamin A, and also have the functions of anti-cancer, increasing immunity, and delaying aging. Lycopene ε-cyclase is found only in plants and is used for the synthesis of δ-carotene and α-carotene. Recent studies have found that α-carotene has the ability to specifically resist cancer metastasis. Since the accumulation of β-carotene is common in most plants and microorganisms, the proportion of α-carotene is relatively low. And the existing microbial synthesis is mainly aimed at β-carotene, and there is no effective synthesis of α-carotene in microorganisms. Banana fruit contains a large amount of α-carotene and β-carotene....

AI Technical Summary

Problems solved by technology

Since the accumulation of β-carotene is common in most plants and microorganisms, the proportion of α-carotene is relatively low

And the existing microbial synthesis is mainly aimed at β-carotene, and there is no effective synthesis of α-carotene in microorganisms. Banana fruit contains a large amount of α-carotene and β-carotene. The production of α-carotene and other carotenoids by lycopene ε-cyclase is a relatively safe and effective way

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