Composite microbial fertilizer for control of botrytis cinerea and preparation method thereof
A technology of complex microbial bacteria and tomato gray mold, applied in fertilizer mixtures, organic fertilizers, fertilization devices, etc., can solve problems such as inability to achieve control effects, and achieve super-strong control of tomato gray mold, low use, and multiple antagonistic substances Effect
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Embodiment 1
[0036] A compound microbial fertilizer for preventing and treating gray mold of tomato, characterized in that the raw material ratio of the microbial fertilizer is: 20 parts of Streptomyces viridans fermentation broth, 30 parts of Streptomyces Champawady fermentation broth, Bacillus indica 20 parts of fermented liquid, 20 parts of fermented liquid of Bacillus mogaf, 20 parts of fermented liquid of Trichoderma oospore.
[0037] The preparation method of the compound microbial fertilizer for preventing and treating tomato gray mold comprises the following steps:
[0038] Step 1: Preparation of Streptomyces viridans fermentation broth
[0039] Take out the preservation tube of Streptomyces aeruginosa strains, use Gao's No. 1 solid medium to scratch the plate for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When ...
Embodiment 2
[0064] The antagonism test of the present invention's composite microbial bacterial fertilizer to tomato Botrytis cinerea
[0065] (1) Test time and place: In April 2014, it was carried out in the microbiological laboratory of Foshan Yanhui Biotechnology Co., Ltd.;
[0066] (2) Test method:
[0067] (1) Source of Botrytis cinerea for testing: Botrytis cinerea, the pathogen of Botrytis cinerea, was donated by the Agricultural College of Guangxi University, and the pathogenicity test showed strong pathogenicity;
[0068] (2) Tablet confrontation experiment:
[0069] First Botrytis cinerea is activated on the PDA flat plate, after cultivating for 4 days, in the colony edge area, punch holes with a puncher (6mm) to make a bacterial sheet, transfer the Botrytis cinerea bacterial sheet to the center of another PDA flat plate, Then the composite microbial bacterium liquid prepared in the embodiment 1 step (1) is diluted 100 times, and the point is connected at 2.5 centimeters away ...
Embodiment 3
[0075] Experimental Effect of Compound Microbial Fertilizer for Controlling Botrytis cinerea in Greenhouse
[0076] (1) Test treatment:
[0077] Test group: the compound microbial bacterial fertilizer prepared in Example 1 is spraying each 4L / mu on the leaves in the method of preventing and treating tomato gray mold, and its dilution factor is 100-200 times, and it is used continuously for 2 times, every 7 days use once;
[0078] Chemical agent: azoxystrobin suspension concentrate (250 g / L) (Syngenta Co., Ltd., UK), diluted 500 times with water;
[0079] Blank control: clear water
[0080] (2) Test method: From March to July 2015, it was carried out in a tomato greenhouse in Cang County, Cangzhou City, Hebei Province. Botrytis cinerea occurred moderately on the tomato leaves in the whole shed at the beginning of the experiment, and the diseased leaves were removed before spraying. Three rows per plot, four replicates, arranged in random blocks. A knapsack electric sprayer...
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