Composite microbial fertilizer for control of botrytis cinerea and preparation method thereof

A technology of complex microbial bacteria and tomato gray mold, applied in fertilizer mixtures, organic fertilizers, fertilization devices, etc., can solve problems such as inability to achieve control effects, and achieve super-strong control of tomato gray mold, low use, and multiple antagonistic substances Effect

Inactive Publication Date: 2017-02-15
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the diversity and synchronous evolution of pathogenic bacteria, a single antagonistic strain cannot achieve an ideal control effect, so it is necessary to find a combination of different types of bacteria to form a combination with better control effect against Botrytis cinerea
Due to the diver

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A compound microbial fertilizer for preventing and treating gray mold of tomato, characterized in that the raw material ratio of the microbial fertilizer is: 20 parts of Streptomyces viridans fermentation broth, 30 parts of Streptomyces Champawady fermentation broth, Bacillus indica 20 parts of fermented liquid, 20 parts of fermented liquid of Bacillus mogaf, 20 parts of fermented liquid of Trichoderma oospore.

[0037] The preparation method of the compound microbial fertilizer for preventing and treating tomato gray mold comprises the following steps:

[0038] Step 1: Preparation of Streptomyces viridans fermentation broth

[0039] Take out the preservation tube of Streptomyces aeruginosa strains, use Gao's No. 1 solid medium to scratch the plate for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When ...

Embodiment 2

[0064] The antagonism test of the present invention's composite microbial bacterial fertilizer to tomato Botrytis cinerea

[0065] (1) Test time and place: In April 2014, it was carried out in the microbiological laboratory of Foshan Yanhui Biotechnology Co., Ltd.;

[0066] (2) Test method:

[0067] (1) Source of Botrytis cinerea for testing: Botrytis cinerea, the pathogen of Botrytis cinerea, was donated by the Agricultural College of Guangxi University, and the pathogenicity test showed strong pathogenicity;

[0068] (2) Tablet confrontation experiment:

[0069] First Botrytis cinerea is activated on the PDA flat plate, after cultivating for 4 days, in the colony edge area, punch holes with a puncher (6mm) to make a bacterial sheet, transfer the Botrytis cinerea bacterial sheet to the center of another PDA flat plate, Then the composite microbial bacterium liquid prepared in the embodiment 1 step (1) is diluted 100 times, and the point is connected at 2.5 centimeters away ...

Embodiment 3

[0075] Experimental Effect of Compound Microbial Fertilizer for Controlling Botrytis cinerea in Greenhouse

[0076] (1) Test treatment:

[0077] Test group: the compound microbial bacterial fertilizer prepared in Example 1 is spraying each 4L / mu on the leaves in the method of preventing and treating tomato gray mold, and its dilution factor is 100-200 times, and it is used continuously for 2 times, every 7 days use once;

[0078] Chemical agent: azoxystrobin suspension concentrate (250 g / L) (Syngenta Co., Ltd., UK), diluted 500 times with water;

[0079] Blank control: clear water

[0080] (2) Test method: From March to July 2015, it was carried out in a tomato greenhouse in Cang County, Cangzhou City, Hebei Province. Botrytis cinerea occurred moderately on the tomato leaves in the whole shed at the beginning of the experiment, and the diseased leaves were removed before spraying. Three rows per plot, four replicates, arranged in random blocks. A knapsack electric sprayer...

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PUM

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Abstract

The invention discloses a composite microbial fertilizer for control of botrytis cinerea and a preparation method thereof. The composite microbial fertilizer is prepared by mixing of the following raw materials: a Str. Viridifaciens fermentation broth, a S. champavatii fermentation broth, an India bacillus fermentation broth, a B. mojavensis fermentation broth, and a Trichoderma ovalisporium fermentation broth. The strains involved in the invention can comprehensively utilize the antibiosis, competition, hyperparasitism and bacteriolysis effects between or within microbial species, or utilize secondary metabolites to induce the disease resistance of tomatoes, and can generate a variety of antibiotics like tetracycline, 7-aureomycin, champamycin A and B (antifungal antibiotics of heptaene) and champavatin to inhibit botrytis cinerea pathogenic bacteria and strengthen its disease prevention effect. The composite microbial fertilizer provided by the invention is safe to human and livestock, is environment-friendly, and does not produce drug resistance when applied to control of botrytis cinerea. The composite microbial fertilizer has the advantages of simple preparation method, low cost, and easy use.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control and microbial fertilizer, and in particular relates to a compound microbial fertilizer for preventing and treating tomato gray mold and a preparation method thereof. Background technique [0002] Botrytis cinerea, as a fungal disease that harms tomato production in modern cultivation, often causes huge losses. Tomatoes in some protected areas in our country are seriously damaged by gray mold, and the yield reduction is as high as 60% or more; many protected tomato areas are damaged by gray mold, resulting in a 20%-30% reduction in yield, and more than 50% in severe areas; tomatoes in Jiaodong, Shandong Province The young fruit is affected by Botrytis cinerea, and the yield is reduced by 10%-30%, and the yield is even severe in severe plots. [0003] Botrytis cinerea is a worldwide fungal disease caused by Botrytis cinerea, which seriously affects the yield and quality of t...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F11/08
CPCC05F11/08C05G3/00Y02A40/22
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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