Rolling circle constant-temperature amplification quick detection primer and kit for mycoplasma pneumoniae

Abstract

The invention belongs to the field of microbiological detection, and particularly relates to a rolling circle constant-temperature amplification quick detection primer and a kit for mycoplasma pneumonia. The primer is designed according to a specific conservative target sequence of a mycoplasma pneumonia P1 gene; the primer consists of four primers, including a front end inner primer SIP, a rear end inner primer ASIP, and outer primers S and AS; in the pair of the outer primers S and AS, the S primer is positioned on the upstream of the SIP, and the AS primer is positioned on the downstream of the ASIP. The rolling circle constant-temperature amplification quick detection primer and the kit for the mycoplasma pneumonia can be used for quick detection of the mycoplasma pneumonia, provide guarantee for clinical early diagnosis and treatment, and realize an important value of quick, accurate, convenient and low-cost detection.

Description

technical field [0001] The invention belongs to the field of microorganism detection, in particular to a primer and a kit for rapid detection of rolling circle constant temperature amplification of mycoplasma pneumoniae. Background technique [0002] Mycoplasma pneumoniae (Mycoplasma pneumoniae, Mp) is one of the most common pathogens of respiratory tract infection. Epidemiological surveys in my country show that Mp has become the primary pathogenic bacteria of adult community-acquired pneumonia in my country. Mp infection can not only cause respiratory diseases, but also systemic multi-organ lesions, such as encephalitis, myocarditis, hepatitis, nephritis, immune hemolytic anemia, etc., and the number of critical cases caused by Mp has been increasing in recent years. Due to the lack of cell wall of Mp, the effect of commonly used antibiotics such as β-lactams and other drugs is not obvious. [0003] The existing methods for detecting Mp in the medical field are: Mycoplas...

AI Technical Summary

Problems solved by technology

Due to the lack of cell wall of Mp, the treatment with commonly used antibiotics such as β-lactams is not effective

[0003] The existing methods for detecting Mp in the medical field are: Mycoplasma pneumoniae culture, Mycoplasma pneumoniae nucleic acid PCR amplification and serological detection (IgM and IgG). These methods are time-consuming, require special instruments, and are costly.