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Tooth specimen preservation liquid and application thereof and tooth specimen preservation method

A technology for preserving liquids and specimens, applied in the biological field, can solve the problem of unsatisfactory reduction of bacterial contamination, and achieve the effect of reducing the degree of bacterial contamination of cells, preservation and transportation protection

Inactive Publication Date: 2017-02-22
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned preservation solution can effectively maintain the activity of dental pulp stem cells and reduce bacterial contamination to a certain extent, but the degree of reduction in bacterial contamination is still not ideal, and more excellent preservation solutions need to be provided to increase the degree of protection of dental specimens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: preparation preservation solution of the present invention

[0025] 8g sodium chloride (NaCl), 0.2g potassium chloride (KCl), 1.44g disodium hydrogen phosphate (NaCl 2 HPO 4 ), 0.24g potassium dihydrogen phosphate (KH 2 PO 4 ), adjust the pH to 7.2, and set the volume to 1L; then sterilize by high-pressure steam, take 10mL of the solution and add penicillin to make the final concentration 100U / mL, add streptomycin to make the final concentration 100μg / mL, add amphotericin B, Make the final concentration 1 μg / ml, and store at 4°C for later use.

Embodiment 2

[0026] Embodiment 2: preparation preservation solution of the present invention

[0027] 8g sodium chloride (NaCl), 0.2g potassium chloride (KCl), 1.44g disodium hydrogen phosphate (NaCl 2 HPO 4 ), 0.24g potassium dihydrogen phosphate (KH 2 PO 4 ), adjust the pH to 7.2, and set the volume to 1L; then autoclave, take 10mL of the solution and add penicillin to make the final concentration 50U / mL, add streptomycin to make the final concentration 500μg / mL, add amphotericin B, Make the final concentration 0.5 μg / ml, and store it at 4°C for later use.

Embodiment 3

[0028] Embodiment 3: preparation preservation solution of the present invention

[0029] 8g sodium chloride (NaCl), 0.2g potassium chloride (KCl), 1.44g disodium hydrogen phosphate (NaCl 2 HPO 4 ), 0.24g potassium dihydrogen phosphate (KH 2 PO 4 ), adjust the pH to 7.2, and set the volume to 1L; then sterilize by high-pressure steam, take 10mL of the solution and add penicillin to make the final concentration 500U / mL, add streptomycin to make the final concentration 50μg / mL, add amphotericin B, Make the final concentration 5 μg / ml, and store it at 4°C for later use.

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Abstract

The invention relates to the technical field of biology, and discloses tooth specimen preservation liquid and application thereof and a tooth specimen preservation method. The preservation liquid comprises sodium chloride, potassium chloride, disodium hydrogen phosphate, monopotassium phosphate, penicillin, streptomycin and amphotericin B. Sodium chloride, potassium chloride, disodium hydrogen phosphate, monopotassium phosphate, penicillin, streptomycin and amphotericin B are selected to form the novel tooth specimen preservation liquid; compared with existing preservation liquid, the preservation liquid can provide a living environment more suitable for dental pulp stem cells, the activity of the dental pulp stem cells is not influenced, meanwhile, the contamination degree of bacteria on the cells is greatly reduced, and effective protection is provided on the aspects of preservation and transportation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tooth specimen preservation solution and its application and a tooth specimen preservation method. Background technique [0002] Dental pulp stem cells (DPSCs) are a kind of mesenchymal stem cells with self-renewal, strong proliferation ability and multi-lineage differentiation ability existing in dental pulp tissue. Dental pulp stem cells have the potential of multi-directional differentiation. In addition to cells capable of forming mineralized nodules, they can also differentiate into fat, bone, cartilage, muscle, vascular endothelial, liver, nerve and other cells after being induced by different cytokines Department type. [0003] To successfully extract a sufficient amount of standard dental pulp stem cells from isolated teeth, the first problem encountered is the preservation of isolated teeth. The environment of the oral cavity is different from that of other tissues. Ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215
Inventor 葛啸虎陈海佳王一飞冯德龙王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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