A test tube with a coaxial rotary valve

Abstract

The invention discloses a test tube with a coaxial rotary valve. The test tube comprises a first tube body, and further comprises a second tube body, wherein a bottom region is arranged below the first tube body; a flow guide hole is formed in the bottom region; the second tube body sleeves the first tube body, and comprises a sealing part capable of being matched and sealed with the flow guide hole; after the second tube body and the first tube body rotate for a certain angle along the axial direction, switching of opening and closing of the flow guide hole is realized. The test tube with the coaxial rotary valve, provided by the invention, has the beneficial effects that the one rotary valve (tube body) is additionally arranged at the bottom of an existing test tube, a solution in the tube is removed from the bottom through a vacuum sucking manner after the valve is switched on, and a new solution is added from an opening at the top; in a whole process, a plurality of gun heads can be saved and automatic operation is easy to realize.

Description

technical field [0001] The present invention relates to the technical field of sample analysis test tubes used in the detection or analysis of biological reactions, in particular, to a test tube with a coaxial rotary valve that can be used for protein or DNA analysis. Background technique [0002] At present, in the field of molecular biology, PCR reaction is a commonly used experimental technique. PCR reaction, namely polymerase chain reaction (PCR), is a method of enzymatically synthesizing specific DNA fragments in vitro. Several steps such as annealing and extension at suitable temperature form a cycle, and the cycle is carried out, so that the target DNA can be rapidly amplified, and has the characteristics of strong specificity, high sensitivity, simple operation, and time saving. It can be used not only for basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also for disease diagnosis. PCR technology is also known as cell-free molec...

AI Technical Summary

Problems solved by technology

[0003] The existing PCR plate includes a shaped plate made of PC material and a tube sheet made of PP material. The tube plate is provided with a plurality of reaction tubes. The shaped plate and the tube sheet are in a split structure. On the end surface of the plate, there are several holes on the tube plate, and the shaped plate is provided with bumps matching the holes, and the tube plate and the shaped plate are fixed by the tight fit between the bumps and the holes, but this fixing structure is relatively Complicated, cumbersome to assemble, low production efficiency

At the same time, the existing reaction tube (centrifuge tube) is a tubular container with a cover. When the solution is added to the tube, the solution must be discarded by suction. In the automatic extraction of genes, it is necessary to add and discard multiple solutions, which leads to Repeated suction and discard actions are also for the purpose of preventing cross-contamination of the solution. Each time consumption of multiple pipette tips brings a certain loss and waste, and it is difficult to realize automatic operation

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