A method for rapid detection of residual acrylamide monomer content in cationic polyacrylamide
A technology of acrylamide monomer and polyacrylamide, which is applied in the direction of measuring devices, material separation, instruments, etc., can solve the problems that enterprises cannot quickly detect products, complicated pre-processing, and long detection time, so as to ensure accuracy, Shorten the extraction time and meet the effect of rapid detection
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Embodiment 1
[0037] Instruments and Conditions
[0038] High performance liquid chromatography: Shimadzu LC-15C;
[0039] Chromatographic column: Wondasil C18 (4.6×250mm, 0.5μm) chromatographic column;
[0040] Mobile phase volume ratio: NaH 2 PO 4 Aqueous solution: Methanol=90:10, pH=3, NaH 2 PO 4 The concentration of the aqueous solution is 20mM;
[0041] Detection wavelength: 220nm;
[0042] Flow rate: 1.5mL / min;
[0043] Injection volume: 20μL;
[0044] Column temperature: 40°C.
[0045] Weigh 0.1g of acrylamide in a beaker, accurate to 0.1mg, dissolve it in ethanol and dilute it in a 100mL volumetric flask. This solution is a 1000mg / L acrylamide standard solution. 50mg / L, 20mg / L, 10mg / L, 8mg / L, 6mg / L, 4mg / L, 2mg / L standard solutions with 8 serial concentrations, injected into the high performance liquid chromatograph in parallel for three times to get the average value of the peak area , and draw an acrylamide standard curve, such as figure 1 As shown, the linear equation i...
Embodiment 2
[0059] Instruments and Conditions
[0060] High performance liquid chromatography: Shimadzu LC-15C;
[0061] Chromatographic column: Wondasil C18 (4.6×250mm, 0.5μm) chromatographic column;
[0062] Mobile phase volume ratio: NaH 2 PO 4 Aqueous solution: Methanol=90:10, pH=3, NaH 2 PO 4 The concentration of the aqueous solution is 20mM;
[0063] Detection wavelength: 220nm;
[0064] Flow rate: 1.5mL / min;
[0065] Injection volume: 20μL;
[0066] Column temperature: 40°C.
[0067] Weigh samples 1-9 of the same batch of cationic polyacrylamide in a mixed solvent of 50mL ethanol and water. Filter and inject liquid chromatograph respectively, inject samples according to the above method, and get the average value of the peak area of parallel injection three times, read the concentration of residual acrylamide monomer in the extract according to the standard curve in Example 1, and according to the implementation The formula for calculating the mass fraction ω of residua...
Embodiment 3
[0074] Instruments and Conditions
[0075] High performance liquid chromatography: Shimadzu LC-15C;
[0076] Chromatographic column: Wondasil C18 (4.6×250mm, 0.5μm) chromatographic column;
[0077] Mobile phase volume ratio: KH 2 PO 4 Aqueous solution: methanol = 80:20, pH = 3.5, KH 2 PO 4 The concentration of the aqueous solution is 10mM;
[0078] Detection wavelength: 210nm;
[0079] Flow rate: 1.0mL / min;
[0080] Injection volume: 5μL;
[0081] Column temperature: 40°C.
[0082] Weigh 0.1g of acrylamide in a beaker, accurate to 0.1mg, dissolve it with acetonitrile and dilute it in a 100mL volumetric flask to prepare a 1000mg / L acrylamide standard solution, use this standard solution to prepare 1000mg / L, 500mg / L L, 100mg / L, 50mg / L, 10mg / L, 0.5mg / L standard solution of 6 serial concentrations are injected into the high performance liquid chromatograph, the sample is injected according to the above method, and the average value of the peak area is obtained by parallel...
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