Method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii

A technology of Luo Han Guo total saponins and Luo Han Guo, applied in the direction of chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of limiting the application of natural sweeteners and affecting the taste of Luo Han Guo total saponins, and achieve the effect of pure sweetness

Inactive Publication Date: 2017-02-22
桂林益天成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the total saponins of Momordica grosvenori produced by the above method contain mogrosides, resulting in a strong after-bitterness in the extract, which affects the mouthfeel of total saponins of Momordica grosvenori and limits its application as a natural sweetener

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) adding grosvenoria grosvenori to 40% ethanol with a volume concentration 5 times its weight and reflux extracting for 3 times, extracting for 1 hour each time, combining the extracts, filtering, taking the filtrate to recover ethanol, and obtaining extract A;

[0028] 2) Add D201 macroporous resin to D201 macroporous resin after adding 8 times its weight in water to dissolve the extract A. The flow rate of the solution on the column is 0.3BV / h, and it is first eluted with water until it is colorless. Elute with ethanol with a volume concentration of 75%, and the elution rate of ethanol is 0.5BV / h, collect the ethanol eluate, recover ethanol from the eluate, and obtain extract B;

[0029] 3) Add the extract B to 10 times its weight in water to dissolve, put D101+ macroporous resin on the solution, the column flow rate of the solution is 0.3BV / h, and water is eluted, the water elution flow rate is 0.5BV / h, and the collected water is eluted liquid, concentrated and drie...

Embodiment 2

[0031] 1) adding 10 times the weight of Luo Han Guo to ethanol with a volume concentration of 50% and refluxing extraction for 3 times, each extraction for 2 hours, combining the extracts, filtering, taking the filtrate to recover ethanol, and obtaining extract A;

[0032] 2) Add D201 macroporous resin to the extract A by adding 15 times its weight in water to dissolve it. The flow rate of the solution on the column is 0.5BV / h. Elute with ethanol with a volume concentration of 80%, the elution rate of ethanol is 0.6BV / h, collect the ethanol eluate, recover ethanol from the eluate, and obtain extract B;

[0033] 3) Add extract B to 15 times its weight in water to dissolve, put D101+ macroporous resin on the solution, the column flow rate of the solution is 0.5BV / h, and water is eluted, the water elution flow rate is 0.6BV / h, and the collected water is eluted liquid, concentrated and dried to obtain the total saponins of Monk Fruit. After tasting, the total saponins of Luo Han ...

Embodiment 3

[0035] 1) adding grosvenoria grosvenori to 8 times the weight of ethanol with a volume concentration of 45% and refluxing extraction for 3 times, each extraction for 1.5 hours, combining the extracts, filtering, taking the filtrate to recover ethanol, and obtaining extract A;

[0036] 2) Add the extract A to 12 times its weight in water to dissolve it, and apply it to D201 macroporous resin. The flow rate of the solution on the column is 0.4BV / h, and it is first eluted with water until it is colorless. The elution flow rate of water is 1.5BV / h. Then elute with ethanol with a volume concentration of 78%, the elution rate of ethanol is 0.55BV / h, collect the ethanol eluate, recover ethanol from the eluate, and obtain extract B;

[0037] 3) Add the extract B to 12 times its weight in water to dissolve, put D101+ macroporous resin on the solution, the column flow rate of the solution is 0.4BV / h, and water is eluted, the water elution flow rate is 0.55BV / h, and the collected water is...

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Abstract

The invention discloses a method for extracting siraitia grosvenorii total saponin in siraitia grosvenorii. The method includes the following steps that firstly, siraitia grosvenorii is added into ethyl alcohol to be extracted, an extraction solution is filtered, ethyl alcohol in the extraction solution is recycled, and an extract A is obtained; secondly, the extract A is added into water to be dissolved, then D201 macroporous resin is added, the mixture is eluted with water to be colorless and then eluted with ethyl alcohol, an ethyl alcohol elution solution is collected, ethyl alcohol is recycled from the elution solution, and an extract B is obtained; thirdly, the extract B is added into water to be dissolved, D101+ macroporous resin is added, the mixture is eluted with water, an elution solution is collected, concentrated and dried, and siraitia grosvenorii total saponin is obtained. Compared with the prior art, the siraitia grosvenorii total saponin extracted through the method does not contain bitter substances and is pure in sweetness and free of rebitter.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting total saponins of Luo Han Guo from Luo Han Guo. Background technique [0002] Luo Han Guo Siraitia grosvenorii is the fruit of the genus Luo Han Guo in the family Cucurbitaceae of Dicotyledonae, mainly produced in Guangxi, Guangdong, Hunan, Jiangxi and other places. The dried fruit of Luo Han Guo is oval or round, with a brown or smoky shell and a large number of small black hairs, slightly larger than goose eggs. The dried fruit is lighter, and the seeds, peel and pulp can be used as medicine. Luo Han Guo has extremely high nutritional value, containing a large amount of carbohydrates, proteins, vitamins, fatty acids, a variety of essential amino acids and trace elements. [0003] Mogroside Ⅰ (sianenoside Ⅰ), mogroside Ⅱ E (eogroside Ⅱ E), mogroside Ⅲ (mogroside Ⅲ), mogroside Ⅲ E (mogroside Ⅲ E), mogroside Ⅳ (ogroside Ⅳ), mogro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00A23L27/30
CPCC07J17/005A23V2002/00
Inventor 谢冬养黄建军陆美珍叶出良
Owner 桂林益天成生物科技有限公司
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