Lycopene beta-cyclase gene promoter and application

A lycopene and promoter technology, applied in the field of genetic engineering, can solve the problem of specific side effects of host cells, etc., and achieve the effect of small side effects and strong specificity

Inactive Publication Date: 2017-02-22
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Based on this, it is necessary to provide a specific promoter for knocking out the lycopene β-cyclase gene in tomato flowers and fruits in view of the problems that traditional RNAi vectors are prone to side effects on host cells and have poor specificity. child and application

Method used

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  • Lycopene beta-cyclase gene promoter and application
  • Lycopene beta-cyclase gene promoter and application
  • Lycopene beta-cyclase gene promoter and application

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Embodiment Construction

[0026] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of ...

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Abstract

The invention relates to a Lycopene beta-cyclase gene promoter and application. The sequence of the Lycopene beta-cyclase gene promoter is shown as the SEQ ID NO. 1. The LcyB1 gene 5'end upstream promoting sub-region sequence is firstly cloned in a tomato, the promoter can be used for constructing a gene expression kit, an expression vector and tomato plant cells and applied in a tomato plant for cultivating Lycopene, compared with traditional expression vectors, higher specificity is provided, and the promoter comes from the tomato so that small side effect is caused to the tomato plant.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a lycopene β-cyclase gene promoter and its application. Background technique [0002] Lycopene (Lycopene) has multiple functions such as quenching singlet oxygen, scavenging free radicals, inducing intercellular communication, regulating cell proliferation, etc., especially the quenching effect on superoxide free radicals in the living body and on certain cancer The inhibitory effect on cell proliferation is stronger than α-carotene and β-carotene, so it has become one of the most concerned carotenoid pigments. It is the focus of international functional food research and cosmetics and food additives research. It is expected to become An important chemical anticancer substance, which is of great significance to human health. The human body cannot synthesize lycopene by itself, and due to the limitation of the distribution of lycopene in nature, the main source of lycopene for ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC12N9/90C12N15/8218C12Y505/01019
Inventor 莫爱琼万小荣
Owner ZHONGKAI UNIV OF AGRI & ENG
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