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Preparation method for sapindoside products

A technology of Sapindus japonica and Sapindus japonica, applied in the field of preparing Sapindus japonica saponin products, can solve problems such as the inability to meet the needs of development and production of Sapindus japonica medicinal products, and achieve the advantages of inhibiting tumor growth, easy recovery, and non-toxic solvent. Effect

Inactive Publication Date: 2017-03-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only providing Sapindia saponins and its in vitro tumor inhibition data cannot meet the needs of the development and production of Sapindus pharmaceutical products

Method used

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  • Preparation method for sapindoside products
  • Preparation method for sapindoside products
  • Preparation method for sapindoside products

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preparation example Construction

[0027] A preparation method of Sapindus saponin product with mouse breast cancer inhibitory effect, comprising the following steps:

[0028] Firstly, the impurities in the water extract of Sapindus chinensis are removed, and the present invention adopts an ethyl acetate extraction method to remove impurities. Specifically, carry out liquid-liquid extraction on the water extract of Sapindus chinensis with ethyl acetate, remove the oil phase, that is, the ethyl acetate phase, and retain the water phase in the obtained extract. The volume ratio of ethyl acetate to the extract depends on the concentration of components in the aqueous extract of Sapindus chinensis, preferably the volume ratio of ethyl acetate to the extract is 0.5-5:1, more preferably 1-2:1.

[0029] In the present invention, the ethyl acetate extraction preferably removes impurities twice, but it is not limited to two times, and the number of extractions can be increased or decreased depending on the extraction ef...

Embodiment 1

[0041] Embodiment 1: the preparation of sapindus saponin product

[0042] 1) Ethyl acetate extraction to remove impurities: Add 10L Sapindus sapinsinum water extract to 10L ethyl acetate for liquid-liquid extraction, let it stand until it is completely divided into two layers, discard the ethyl acetate phase, take the water phase (about 8L) and Add 8L of ethyl acetate for re-extraction, take the water phase, remove the ethyl acetate by evaporation under reduced pressure at a temperature of 40°C and a pressure of 0.01MPa, and obtain 5L of the water phase from which the ethyl acetate has been removed;

[0043] 2) Macroporous adsorption resin to remove sugars: put the above-mentioned water phase from which ethyl acetate has been removed on a 10LAB-8 macroporous adsorption resin column at a flow rate of 20mL / min, and first elute with deionized water until the eluent is free. color, discard the eluent; then continue to elute with 30% ethanol aqueous solution, collect the eluate unt...

Embodiment 2

[0046] Embodiment 2: the preparation of sapindus saponin product

[0047] 1) Ethyl acetate extraction to remove impurities: Add 10L Sapindus sapinsinum water extract to 10L ethyl acetate for liquid-liquid extraction, let it stand until it is completely divided into two layers, discard the ethyl acetate phase, take the water phase (about 8L) and Add 8L of ethyl acetate for re-extraction, take the water phase, remove the ethyl acetate by evaporation under reduced pressure at a temperature of 45°C and a pressure of 0.005MP, and obtain 5L of the water phase from which the ethyl acetate has been removed;

[0048] 2) Macroporous adsorption resin to remove sugars: put the above-mentioned water phase from which ethyl acetate has been removed on a 10L macroporous adsorption resin column (X-5 type) at a flow rate of 10mL / min, first elute with deionized water until the elution The liquid was colorless, and the eluent was discarded; then, elution was continued with 90% ethanol aqueous sol...

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Abstract

The invention relates to a preparation method for sapindoside products with inhibitory effect on the breast cancer of mice. The preparation method comprises the following steps: carrying out extraction with ethyl acetate for impurity removal two times, leaving a water phase, subjecting the water phase to pressure-reduced evaporation, carrying out adsorption with a resin column, then carrying out elution with water to a colorless state and discarding eluate; then carrying out elution with ethanol with a concentration of 30 to 100% to a colorless state, collecting eluate and thoroughly volatilizing ethanol; allowing the eluate to pass through a MCI column, carrying out elution with water to a colorless state and then carrying out pressure-reduced evaporation and spray drying so as to obtain a graded Chinese soapberry substance 1; and carrying out elution with ethanol with a concentration of 20 to 40% to a colorless state, then carrying out elution with ethanol with a concentration of 50 to 100% to a colorless state, collecting eluate and then subjecting the eluate to pressure-reduced evaporation and spray drying so as to obtain a graded Chinese soapberry substance 2. The prepared sapindoside products can selectively inhibit in-vitro growth of breast cancer cells at a low concentration without influence on growth of normal breast cells, wherein an inhibition rate is about 100%; and in-vivo test results also show that the sapindoside products have strong inhibitory effect on the breast cancer of mice, and the inhibition rates reach 40.22% and 38.77%, respectively.

Description

technical field [0001] The method relates to a technology for preparing sapindus saponins, in particular to a method for preparing sapindus saponins which have inhibitory effect on mouse breast cancer. Background technique [0002] Sapindus mukorossi Gaerth. tree is a perennial woody oil-bearing plant, which has the characteristics of strong adaptability, fast growth, and high oil production; as early as the Ming Dynasty, Li Shizhen's "Compendium of Materia Medica" recorded its medicine use method. Sapindus fruit is rich in saponins and sesquiterpene glycosides, which have biological activities such as sterilization, anti-inflammatory and anti-tumor. In addition, the roots, leaves, and seeds of Sapindus chinensis have medicinal effects and are rare natural resources, so they have attracted the attention of researchers. [0003] At present, the research on the anti-tumor activity of the extract of Sapindus chinensis is not perfect, and most of them stay in the stage of in v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61P35/00
CPCA61K36/77
Inventor 姚卫蓉
Owner JIANGNAN UNIV
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