Serum-free medium and method for culturing mesenchymal stem cell
A serum-free medium and stem cell technology, applied in the field of stem cell culture, can solve the problems of high cost and unsatisfactory effect of mesenchymal stem cell culture, and achieve the goal of improving safety, high scientific research and medical application value, and increasing value-added efficiency. Effect
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Embodiment 1
[0034] 1. Formula
[0035]
[0036]
[0037] 2. Preparation
[0038] Weigh each component, dissolve 10g of L-DMEM dry powder in 800mL of ultrapure water, then add the remaining components to it, use a magnetic stirrer at room temperature to fully dissolve it, add ultrapure water to 1L, and then Filter with a 0.22 μm pore size filter and store at 4°C for later use.
Embodiment 2
[0040] 1. Formula
[0041]
[0042]
[0043] 2. Preparation
[0044] Weigh each component, dissolve 10g of DMEM / F12 dry powder in 800mL of ultrapure water, then add the rest of the components to it, use a magnetic stirrer at room temperature to fully dissolve it, add ultrapure water to 1L, and then Filter with a 0.22 μm pore size filter and store at 4°C for later use.
Embodiment 3
[0046] 1. Formula
[0047]
[0048]
[0049] 2. Preparation
[0050] Weigh each component, dissolve 10g of L-DMEM dry powder in 800mL of ultrapure water, then add the remaining components to it, use a magnetic stirrer at room temperature to fully dissolve it, add ultrapure water to 1L, and then Filter with a 0.22 μm pore size filter and store at 4°C for later use.
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