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Serum-free medium and method for culturing mesenchymal stem cell

A serum-free medium and stem cell technology, applied in the field of stem cell culture, can solve the problems of high cost and unsatisfactory effect of mesenchymal stem cell culture, and achieve the goal of improving safety, high scientific research and medical application value, and increasing value-added efficiency. Effect

Inactive Publication Date: 2017-03-08
深圳江淼医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the object of the present invention is to provide a serum-free medium and method for culturing mesenchymal stem cells, which are used to solve the problems of unsatisfactory mesenchymal stem cell culture and high cost in the prior art

Method used

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  • Serum-free medium and method for culturing mesenchymal stem cell
  • Serum-free medium and method for culturing mesenchymal stem cell
  • Serum-free medium and method for culturing mesenchymal stem cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Formula

[0035]

[0036]

[0037] 2. Preparation

[0038] Weigh each component, dissolve 10g of L-DMEM dry powder in 800mL of ultrapure water, then add the remaining components to it, use a magnetic stirrer at room temperature to fully dissolve it, add ultrapure water to 1L, and then Filter with a 0.22 μm pore size filter and store at 4°C for later use.

Embodiment 2

[0040] 1. Formula

[0041]

[0042]

[0043] 2. Preparation

[0044] Weigh each component, dissolve 10g of DMEM / F12 dry powder in 800mL of ultrapure water, then add the rest of the components to it, use a magnetic stirrer at room temperature to fully dissolve it, add ultrapure water to 1L, and then Filter with a 0.22 μm pore size filter and store at 4°C for later use.

Embodiment 3

[0046] 1. Formula

[0047]

[0048]

[0049] 2. Preparation

[0050] Weigh each component, dissolve 10g of L-DMEM dry powder in 800mL of ultrapure water, then add the remaining components to it, use a magnetic stirrer at room temperature to fully dissolve it, add ultrapure water to 1L, and then Filter with a 0.22 μm pore size filter and store at 4°C for later use.

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Abstract

The invention belongs to the technical field of stem cells, and particularly relates to a serum-free medium for culturing a mesenchymal stem cell. According to the serum-free medium, a basal culture medium L-DMEM or DMEM / F12 and multiple additives are combined in a customized manner for MSCs in vitro culture, so that ingredients in the culture medium are synergized; the stability and success rate for culturing MSCs in vitro can be greatly improved especially due to application of DKK-1; and the safety of cultured cells in clinical researches is improved by adopting a formula without animal-sourced ingredient. Compared with the prior art, the serum-free medium has the advantages of greatly improving the value adding efficiency of MSCs in vitro culture and maintaining cell growth form of MSCs and cell phenotype stability thereof, provides a novel efficient, stable and safety culture system to MSCs culture in clinical application, and has high scientific researching and medical application values.

Description

technical field [0001] The invention belongs to the technical field of stem cell culture, and in particular relates to a serum-free medium and method for culturing mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of stem cells with high differentiation potential, named for their ability to differentiate into mesenchymal tissues. MSCs mainly exist in connective tissue and organ interstitium, have a high degree of differentiation function and immune regulation function, and can secrete a variety of cytokines. After hematopoietic stem cells, the clinical application of cell therapy has been studied the most, and it is also a relatively mature adult stem cell. . MSCs can differentiate into a variety of mesoderm and neuroectoderm-derived tissue cells, such as osteoblasts, chondrocytes, adipocytes, tenocytes, smooth muscle cells, bone marrow stromal cells, fibroblasts, and various vascular endothelial cells, even nerve cells. Systemi...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N5/0663C12N5/0668C12N2500/12C12N2500/22C12N2500/24C12N2500/30C12N2500/34C12N2500/36C12N2500/38C12N2500/46C12N2500/90C12N2501/105C12N2501/11C12N2501/115C12N2501/135C12N2501/148C12N2501/15C12N2501/235C12N2501/30C12N2501/734C12N2501/998
Inventor 肖佳朱江韩荣飞
Owner 深圳江淼医疗有限公司
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