Method for establishing mycobacterium abscessus RUO database and subtype super map

Abstract

The invention discloses a method for establishing a mycobacterium abscessus RUO database and a subtype super map. The method is characterized by including the following steps of mycobacterium abscessus strain collecting and DNA extracting; 2, gene library preparing and illumine HiSeq sequencing; 3, genome establishing and phylogenetic tree establishing; 4, sample preparing and strain detecting; 5, accuracy confirming. According to the method, mycobacterium abscessus subspecies are analyzed and determined; ionization-flight-time mass-spectrometric-detection subspecies information is desorbed in the mode that a matrix assists in laser, the RUO database and the established super map are established, the sensitivity and the specificity are detected through strain blind testing, the operation procedure and the diagnostic standard for subspecies-level laboratory identification are formulated, a work chain of the microbial standard in-vitro-diagnosis procedure is added, and the efficiency of all microbiological labs on mycobacterium abscessus subtype identification is improved.

Description

technical field [0001] The invention relates to a method, in particular to a method for establishing a Mycobacterium abscessus RUO database and constructing a subtype supermap. Background technique [0002] Mycobacterium abscessus infection has shown an obvious upward trend in recent years. In the past ten years, although the prevention and control of tuberculosis worldwide has brought its transmission and epidemic under control, the incidence of non-tuberculous mycobacteria (NTM) infection has increased rapidly, especially in immunocompromised patients. , and invasive operations such as endoscopy, surgery, and transplantation have increased, and the infection rate of NTM in patients has increased, making it a clinical disease that cannot be ignored. NTM is divided into fast growing type (rapidly growing mycobacteria, RGM) and slow growing type, among which RGM is considered to be more important human pathogenic bacteria. About 65-80% of RGM infections are caused by M. abs...

AI Technical Summary

Problems solved by technology

[0009] These molecular typing methods based on genetic analysis are relatively expensive and take a long time to obtain results, so they are not very practical and difficult to popularize in general microbiological laboratories