Test substance concentration calculation method applied to rapid test strip detection technology

A calculation method and detection technology technology, applied in the field of immunological detection, can solve the problems of affecting concentration calculation, unsatisfactory T/C ratio accuracy, etc., and achieve the effect of improving accuracy

Inactive Publication Date: 2017-03-08
天津博硕科技有限公司
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Problems solved by technology

[0013] In the prior art, there are many methods for obtaining the T / C ratio to calculate the concentration of the analyte, but the accuracy of the obtained T / C ratio is not satisfactory, and even affects the subsequent concentration calculation. This is an urgent need to solve The problem

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  • Test substance concentration calculation method applied to rapid test strip detection technology
  • Test substance concentration calculation method applied to rapid test strip detection technology
  • Test substance concentration calculation method applied to rapid test strip detection technology

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Embodiment Construction

[0057] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0058] The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0059] With the advantages of specificity, sensitivity, speed and simplicity, test strips have attracted more and more attention in the field of detection. It can not only detect qualitatively, but also quantitatively or semi-quantitatively. At present, the most widely used are colloidal gold test strips and fluorescent test strips.

[0060] Colloidal gold test strips use red chloroauric acid as a marker. When using colloidal gold test strips for qualitative testing, visually observe the color changes between the quality control line and the detection line to determine the content of the target test substance; When the paper strip is qualitatively tested, a special test strip image proc...

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Abstract

The invention discloses a test substance concentration calculation method applied to a rapid test strip detection technology. The method comprises the steps as follows: detecting a standard concentration sample to fit a standard concentration curve between T/C and concentration of a target test substance, and acquiring a photograph of the test strip impregnated with the test substance; cutting out the part,containing a fluorescent signal, of the photograph; performing grayscale processing to obtain a two-dimensional array; calculating the average of all the rows (or columns) of the two-dimensional array to obtain a one-dimensional array; removing noise by filtration; eliminating baseline drift; searching for the peak and calculating the peak area ratio T/C; substituting the peak area ratio into the standard concentration curve for conversion of a concentration value. With the adoption of the method, an accurate ratio T/C of the detection band signal T to the quality control band signal C can be obtained, taken as judgment information and substituted into the standard concentration curve, and the accurate concentration of the test substance is obtained, therefore, the accuracy of detection is effectively improved.

Description

technical field [0001] The invention belongs to the field of immunological detection, and in particular relates to a method for calculating the concentration of a test object applied in the rapid detection technology of immunochromatographic test strips. Background technique [0002] Test strip rapid detection technology is a simple, rapid and low-cost immunological detection technology that has gradually developed in recent years. It has a large number of applications in the field of human in vitro diagnostics (IVD, in vitro diagnostic products) and rapid diagnosis of animal diseases, antibody level detection, stimulant residues, pesticide residues, veterinary drug residues and sedative residues rapid detection. [0003] Especially in the field of food safety testing, which has attracted much attention, it is widely used. The rapid detection technology of test strips can complete the detection of food at the food safety supervision site due to its rapid detection and conven...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N21/84
CPCG01N33/558G01N21/8483
Inventor 杜康赵翔王博洪大伟刘新全谢远闫畅孙金康赵建明孙天娇
Owner 天津博硕科技有限公司
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