Test substance concentration calculation method applied to rapid test strip detection technology

A calculation method and detection technology technology, applied in the field of immunological detection, can solve the problems of affecting concentration calculation, unsatisfactory T/C ratio accuracy, etc., and achieve the effect of improving accuracy
CN106483285AInactive Publication Date: 2017-03-08天津博硕科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
天津博硕科技有限公司
Publication Date
2017-03-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a test substance concentration calculation method applied to a rapid test strip detection technology. The method comprises the steps as follows: detecting a standard concentration sample to fit a standard concentration curve between T / C and concentration of a target test substance, and acquiring a photograph of the test strip impregnated with the test substance; cutting out the part,containing a fluorescent signal, of the photograph; performing grayscale processing to obtain a two-dimensional array; calculating the average of all the rows (or columns) of the two-dimensional array to obtain a one-dimensional array; removing noise by filtration; eliminating baseline drift; searching for the peak and calculating the peak area ratio T / C; substituting the peak area ratio into the standard concentration curve for conversion of a concentration value. With the adoption of the method, an accurate ratio T / C of the detection band signal T to the quality control band signal C can be obtained, taken as judgment information and substituted into the standard concentration curve, and the accurate concentration of the test substance is obtained, therefore, the accuracy of detection is effectively improved.
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Description

technical field

[0001] The invention belongs to the field of immunological detection, and in particular relates to a method for calculating the concentration of a test object applied in the rapid detection technology of immunochromatographic test strips. Background technique

[0002] Test strip rapid detection technology is a simple, rapid and low-cost immunological detection technology that has gradually developed in recent years. It has a large number of applications in the field of human in vitro diagnostics (IVD, in vitro diagnostic products) and rapid diagnosis of animal diseases, antibody level detection, stimulant residues, pesticide residues, veterinary drug residues and sedative residues rapid detection.

[0003] Especially in the field of food safety testing, which has attracted much attention, it is widely used. The rapid detection technology of test strips can complete the detection of food at the food safety supervision site due to its rapid detection and conven...

Claims

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