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Pulullan and all-transretinoic acid drug carrier system and preparation method thereof

A technology of all-trans retinoic acid and pullulan, which is applied in the field of pullulan-all-trans retinoic acid drug-loading system and its preparation, can solve the problems of poor stability and poor curative effect of the drug-loading system, and achieve Achieve combined administration and synergistic treatment, overcome drug resistance, and excellent stability

Inactive Publication Date: 2017-03-15
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the above-mentioned shortcomings of the prior art, the object of the present invention is to provide a pullulan-all-trans retinoic acid drug-carrying system and its preparation method, which is used to solve the problem of drug-carrying for treating tumors in the prior art. Problems such as poor system stability and poor curative effect

Method used

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  • Pulullan and all-transretinoic acid drug carrier system and preparation method thereof
  • Pulullan and all-transretinoic acid drug carrier system and preparation method thereof
  • Pulullan and all-transretinoic acid drug carrier system and preparation method thereof

Examples

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Embodiment 1

[0032] A preparation method of a pullulan-all-trans retinoic acid drug-carrying system, comprising the steps of: 1) dissolving 100 mg of pullulan-hydrazine freeze-dried material (amidated pullulan) with 10 mL of deionized water , normal temperature, magnetic stirring to help dissolve, and obtain the first solution;

[0033] 2) Weigh 20 mg of all-trans retinal and add it to the first solution to obtain a mixed solution. Add 2 mL of isopropanol dropwise into the mixed solution until the all-trans retinal is completely dissolved. React under stirring for 12 hours to obtain the second solution;

[0034] 3) Dialyzing the second solution with deionized water to remove small molecular impurities, and freeze-drying to obtain a pullulan-all-trans retinoic acid material;

[0035] 4) Dissolve 20mg of doxorubicin-loaded nanodiamonds in 2mL of deionized water, and dissolve at room temperature with ultrasound to prepare the third solution;

[0036] 5) Dissolve 20 mg of pullulan-all-trans ...

Embodiment 2

[0039] A preparation method of a pullulan-all-trans retinoic acid drug-carrying system, comprising the steps of: 1) dissolving 100mg pullulan-hydrazine freeze-dried material (amidated pullulan) with 20mL deionized water , normal temperature, magnetic stirring to help dissolve, and obtain the first solution;

[0040] 2) Weigh 40 mg of all-trans retinal and add it to the first solution to obtain a mixed solution, drop 7.8 mL of dimethyl sulfoxide into the mixed solution drop by drop until the all-trans retinal is completely dissolved, 1. Reacting for 12 hours under magnetic stirring to prepare the second solution;

[0041] 3) Dialyzing the second solution with deionized water to remove small molecular impurities, and freeze-drying to obtain a pullulan-all-trans retinoic acid material;

[0042] 4) Dissolve 30 mg of doxorubicin-loaded nanodiamond in 2 mL of deionized water, and dissolve at room temperature and ultrasonically to obtain a third solution;

[0043] 5) Dissolve 20 mg...

Embodiment 3

[0046]A preparation method of a pullulan-all-trans retinoic acid drug-carrying system, comprising the steps of: 1) dissolving 100mg pullulan-hydrazine freeze-dried material (amidated pullulan) with 30mL deionized water , normal temperature, magnetic stirring to help dissolve, and obtain the first solution;

[0047] 2) Weigh 60 mg of all-trans retinal and add it to the first solution to obtain a mixed solution. Add 18 mL of isopropanol dropwise into the mixed solution until the all-trans retinal is completely dissolved. React under stirring for 12 hours to obtain the second solution;

[0048] 3) Dialyzing the second solution with deionized water to remove small molecular impurities, and freeze-drying to obtain the pullulan-all-trans retinoic acid material;

[0049] 4) Dissolve 80mg of doxorubicin-loaded nanodiamonds in 7mL of deionized water, and dissolve at room temperature with ultrasonic to prepare the third solution;

[0050] 5) Dissolve 20mg of pullulan-all-trans retinoi...

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Abstract

The invention provides a Pulullan and all-transretinoic acid drug carrier system and a preparation method thereof. The method comprises the following steps of 1) dissolving a Pulullan-hydrazine freeze-drying material by using water, preparing first solution; 2) mixing all-transretinoic retinene and the first solution, adding isopropanol or dimethylsulfoxide to react, and preparing second solution; 3) removing impurities from the second solution, freeze-drying, and preparing a Pulullan and all-transretinoic acid material; 4) dissolving a doxorubicin and nano-diamond compound by using the water, and preparing third solution; 5) dissolving the Pulullan and all-transretinoic acid material prepared in the step 3) in the water, preparing fourth solution, mixing the third solution and the fourth solution to react, and preparing fifth solution; 6) ultra-filtering the fifth solution, and preparing the Pulullan and all-transretinoic acid drug carrier system. The Pulullan and all-transretinoic acid drug carrier system is capable of realizing the double drug carrier of the doxorubicin and the all-transretinoic acid, and improving the stability of the drug carrier system.

Description

technical field [0001] The invention relates to the field of drug carriers, in particular to a pullulan-all-trans retinoic acid drug-loading system loaded with doxorubicin-nanodiamond and a preparation method thereof. Background technique [0002] In recent years, nano-drug delivery systems have made great progress in the treatment of malignant tumors. Paclitaxel-liposomes (Lipusu), paclitaxel-albumin nanoparticles (Abraxane), doxorubicin-liposomes (Gelley), and G-CSF-polyethylene glycol nanoparticles (Lonquex) currently used in clinical practice And nano-drug loading systems such as fluorouracil-polylactic acid nanoparticles (Zhongren Fuan) can improve the efficacy of drugs to a certain extent and reduce toxic and side effects. However, when the nano-drug delivery system is implemented, the tumor is temporarily suppressed, and then often recurs and metastasizes again. [0003] The main reason for this phenomenon is that the existing nano-drug-loaded systems generally have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/61A61K47/69A61K47/04A61K31/704A61P35/00A61K31/203
CPCA61K31/203A61K31/704A61K47/02A61K2300/00
Inventor 李桦楠李发琪涂道月黄楠刘宏英
Owner CHONGQING MEDICAL UNIVERSITY
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