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A cell fixation material suitable for microinjection and its preparation method

A cell fixation and microinjection technology, which is applied in the field of cell fixation materials and preparation, can solve the problems of low efficiency, difficult cell placement and fixation operations, etc., and achieve the effect of easy processing

Inactive Publication Date: 2020-08-21
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a cell fixation method that solves the problems of difficult and low efficiency in placing and fixing cells in microinjection

Method used

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  • A cell fixation material suitable for microinjection and its preparation method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of Cell Fixation Materials Suitable for Microinjection and Fixation Test for Zebrafish Embryo Cells

[0022] combine figure 1 , on a silicon wafer 1 with an area of ​​3.5 cm x 3.5 cm, about 890 x 890 evenly distributed micro-cylindrical pits 2 are uniformly etched; the distance between the centers of adjacent cylinders is 20 microns, the height of the cylinders is 10 microns, and the diameter is 10 microns. Then mix polydimethylsiloxane (PDMS) liquid and curing agent according to the weight ratio of 15:1, then put the processed silicon wafers, put them into a vacuum drying oven together, and put them in a vacuum at 75 degrees Celsius. Let dry for 12 hours. The cured PDMS material is obtained, and after the silicon wafer is peeled off, the PDMS material with a cylindrical structure on the surface is further obtained.

[0023] Zebrafish embryonic cells are approximately 1000 microns in diameter. Use a pipette to absorb a small amount of zebrafish...

Embodiment 2

[0024] Example 2: Preparation of cell fixation materials suitable for microinjection and fixation test for mouse egg cells

[0025] combine figure 1 1700×1700 evenly distributed micro-cylindrical pits 2 are uniformly etched on a silicon wafer 1 with an area of ​​3.5 cm×3.5 cm; the center-to-center distance between adjacent cylinders is 10 microns, the height of the cylinders is 10 microns, and the diameter is 5 microns. Then mix polydimethylsiloxane (PDMS) liquid and curing agent according to the weight ratio of 15:1, then put the processed silicon wafers, put them into a vacuum drying oven together, and put them in a vacuum at 75 degrees Celsius. Let dry for 12 hours. The cured PDMS material is obtained, and after the silicon wafer is peeled off, the PDMS material with a cylindrical structure on the surface is further obtained.

[0026] Zebrafish embryonic cells are approximately 90 microns in diameter. Use a pipette to absorb a small amount of zebrafish embryonic cells 4 ...

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Abstract

The present invention discloses a cell fixation material suitable for microinjection, and a preparation method thereof. The preparation method comprises: uniformly etching pits on a silicon wafer; mixing a polydimethylsiloxane liquid and a curing agent, placing the obtained silicon wafer in the obtained mixture, placing into a vacuum drying oven, and carrying out vacuum drying to obtain a cured material; and stripping the silicon wafer to obtain the polydimethylsiloxane material having the pit structure. According to the present invention, with the cell fixation material, the problems that the cell placement and the fixation operation have the high difficulty and the efficiency is low are solved.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a cell fixation material suitable for microinjection and a preparation method. Background technique [0002] Microinjection is the injection of genes, proteins, or various other organelles into individual cells with an injection needle under an inverted microscope. During the whole injection process, the injection needle will pierce the cells, and the cells cannot move, so the cells need to be fixed. The commonly used fixing method is to use a glass slide to hold the cells or put the cells into a microgroove. However, it is obviously difficult to pick up and move micron-sized cells into the groove or the attachment of the glass slide with a pipette or tweezers under the microscope, which often requires the operator to have extremely high proficiency and consume a lot of time. Moreover, during the clamping process, many cells are easily damaged, resulting in the failure of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/089C08L83/04
CPCC08L83/04C12N11/08
Inventor 谢瑜周赟磊曾凤
Owner XIAMEN UNIV