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Non-invasive method for detecting target molecules

A technology for targeting molecules and subjects, applied in analytical materials, measurement devices, biological tests, etc., can solve problems such as inconvenience and exposure of subjects

Inactive Publication Date: 2017-03-22
CREATICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Invasive techniques for diagnosing GI-related disorders and diseases are inconvenient and expose subjects to significant risk

Method used

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  • Non-invasive method for detecting target molecules
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  • Non-invasive method for detecting target molecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1 - Proteomic Analysis of Sulfate-Based GLF

[0106] In this analysis, the ability of sulfate-based GLFs to support proteome analysis was evaluated. To identify target molecules in GLF obtained using a sulfate-based lavage composition, SUPREP was administered to three human subjects, and proteins in the resulting GLF were analyzed by mass spectrometry. In this example, the GLF is collected from a subject as part of a colonoscopy procedure.

[0107] At the time of collection, whole protease inhibitor tablets (ROCHE) were added and samples were spun at 1000 rpm for 30 minutes at 4°C. Spin the supernatant at 14,000 x g for an additional 30 min to pellet bacteria and debris. 1.8 ml of the supernatant was precipitated with 6 volumes of acetone, extracted with an equal volume of chloroform, and then separated on a C-2 reverse-phase SPE column (Sep-Pak, Waters). Wash the column with 3 column volumes of 0.1% trifluoroacetic acid (TFA), 10%, 20% and 30% acetonitrile...

Embodiment 2

[0120] Example 2 - Proteomic analysis of polyethylene glycol-based GLF

[0121] In this analysis, the ability of polyethylene glycol-based GLFs to support proteome analysis was evaluated. To identify target molecules in GLF obtained using a polyethylene-based lavage composition, two human subjects were administered a polyethylene glycol-based lavage composition and analyzed by mass spectrometry as described in Example 1. Proteins in the resulting GLF. Removal of polyethylene glycol was primarily achieved by chloroform extraction of the lavage fluid. A number of different proteins were identified in GLF from these subjects administered the polyethylene glycol-based lavage composition. Examples of the most abundant identified proteins identified are shown in Table 3, which are consistent with those observed in the preceding tables.

[0122] table 3

[0123]

[0124] In another experiment, a PEG-based douche composition was administered to a subject, and the resulting GL...

Embodiment 3

[0133] Example 3 - Proteome Analysis of Magnesium Citrate-Based GLF

[0134] In this analysis, the ability of magnesium citrate-based GLFs to support proteome analysis was evaluated. To identify target molecules in GLF from human subjects administered a magnesium citrate-based lavage composition, the subject was administered a magnesium citrate-based lavage composition; as part of a colonoscopy procedure, from Subjects collected the GLF. The proteome of the GLF was analyzed by mass spectrometry as described in Example 1. Many different proteins are identified in GLF. Examples of the most abundant identified proteins are listed in Table 6. A number of identified proteins were detected with different colonoscopy preparations, indicating that the proteome is independent of the intestinal preparation used.

[0135] Table 6

[0136]

[0137]

[0138] The foregoing analysis indicated that a large number of target molecules could be detected in samples obtained using mag...

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Abstract

The embodiment of the invention relates to a non-invasive method and composition for collecting, detecting, measuring and identifying target molecules. In some embodiments, the method and composition relate to target molecules in gastrointestinal douche or manure.

Description

[0001] This application is a divisional application of an invention patent application with an application date of September 12, 2011, an application number of 201180074306.6, and an invention title of "Non-invasive method for detecting target molecules". technical field [0002] Embodiments of the invention relate to non-invasive methods and compositions for collecting, detecting, measuring and identifying target molecules. In some embodiments, the methods and compositions relate to target molecules in gastrointestinal lavage fluid (GLF) or feces. [0003] Reference Sequence Listing [0004] This application is filed together with a Sequence Listing in electronic form. The Sequence Listing is provided as a file named USA_007WO.TXT created September 7, 2011, which is approximately 231 KB in size. The information in electronic form of the Sequence Listing is incorporated herein by reference in its entirety. Background technique [0005] Disorders associated with the gastro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574A61K49/00
CPCG01N33/68A61K49/0004G01N33/57488A61B5/425A61B10/0038A61B10/0045A61B2010/0061A61B1/31G01N33/6803
Inventor L·潘内尔J·罗克C·康特拉斯J·迪帕尔马
Owner CREATICS