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A kind of in vitro culture method of Lianxiangshu

A technology for cultivating fragrant trees in vitro, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as no relevant reports in research, and achieve the effect of convenient material collection, sufficient materials, and expanded reproduction coefficient

Active Publication Date: 2018-06-05
CHINA THREE GORGES UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, Mai Miaomiao, Yang Xinchao et al., Zhang Xianyun et al., Chen Rongzhu et al., Du Minhua et al. and Yuan Lijie et al. explored different types and different concentrations of plant growth regulators, as well as different types of explants on the callus induction of Lianxiang tree, The induction of axillary buds, the proliferation of callus and the influence of rooting; but there is no relevant report on the study of somatic embryogenesis

Method used

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  • A kind of in vitro culture method of Lianxiangshu
  • A kind of in vitro culture method of Lianxiangshu
  • A kind of in vitro culture method of Lianxiangshu

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Embodiment 1

[0042] 1 / 2MS Basic Medium: It can be prepared by referring to the literature (Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15: 473-497), only reducing the large amount of elements to the original half of.

[0043] like Figure 9 Among them, the main content of the in vitro culture method of Lianxiangshu is as follows: sterilization—induction of callus—proliferation of callus—induction and proliferation of embryogenic callus—differentiation of somatic embryos—plant Regeneration-transplantation, the specific implementation is as follows:

[0044] 1. Sterilization of cotyledon embryos

[0045] Take the young fruit of Lianxiangshu, wash it with a soft brush in the cleaning solution, rinse it with tap water for 2 hours, and sterilize it with 75% alcohol for 60 seconds on the ultra-clean workbench, 0.1% HgCl 2 Disinfect for 15 min and wash with sterile water 5-6 times. The pericarp was cut longitudinally ...

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Abstract

The invention belongs to the technical field of cell culture and relates to an invitro culture method for cercidiphyllum japonicum. The invitro culture method comprises the steps that a cercidiphyllum japonicum pollinated seed serves as an explant and is placed on a callus inducing medium to induce a callus; the callus proliferates; an embryogenic callus is induced and proliferates; a somatic embryo is differentiated; and a plant is regenerated. According to the invitro culture method, the culture period is 175 days or so, and the survival rate of regenerated plants reaches up to 85%.

Description

technical field [0001] The invention belongs to the technical field of in vitro cultivation of plants, and relates to an in vitro cultivation method of japonica. Background technique [0002] Lianxiangshu (Cerdiphyllum japonicum Sieb.), also known as Shanbaiguo and Yunyishu, is a deciduous tree of the Lianxiang family, with a height of 10-20m and a diameter at breast height of 1m. It is a rare species in a single family and a single genus. All have natural distribution. In China, the distribution of Lianxiang trees can reach Sichuan in the west, Anhui and Zhejiang in the east, and Zhongtiao Mountain in Shanxi in the north. Lianxiang tree is dioecious, the flowers open first or with the leaves, the flowering period is March-April, the fruit matures in September-October, the seeds are oval, and there are oblong transparent spines at the top. Its fragrance is fragrant and can be heard a hundred meters away. It is named Lianxiang tree because the tree shakes and becomes more fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈发菊梁宏伟张德春王玉兵邬洁丽沈祥陵刘文何正权姚伟
Owner CHINA THREE GORGES UNIV