SSR identification method of purity of Chinese cabbage hybrid Yuxin No.55 seeds

An identification method and a technology for Chinese cabbage, applied in the field of vegetable breeding, can solve the problems of deviation of results, long breeding process, long time, etc., and achieve the effects of easy detection, easy analysis, and strong practicability

Inactive Publication Date: 2017-03-29
河南省农业科学院园艺研究所
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Problems solved by technology

[0004] The object of the present invention is to provide the SSR identification method of the seed purity of Chinese cabbage hybrid Yuxin 55, to solve the traditional field morphological observation proposed in the above-mentioned background technology, which requires a long breeding process and is affected by various environments It is also relatively large, often takes a long time and the results may also be biased

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  • SSR identification method of purity of Chinese cabbage hybrid Yuxin No.55 seeds

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Embodiment 1

[0019] The SSR identification method for the seed purity of Chinese cabbage hybrid Yuxin 55, including the steps of material preparation, DNA extraction, genome template stock solution preparation, primer screening and result analysis, the SSR identification method for the seed purity of Chinese cabbage hybrid Yuxin 55, Specific steps are as follows:

[0020] S1: Material preparation: 10 copies of Yuxin 55 Chinese cabbage hybrids were randomly sampled in the field, and SSR primers were artificially synthesized through the published EST sequence information of Chinese cabbage;

[0021] S2: DNA extraction: Use the young leaves on a single Chinese cabbage plant to extract the genomic DNA of Chinese cabbage. When extracting genomic DNA, use a centrifuge tube to buckle 4 young leaf disks the size of the centrifuge tube cap, and pour them into each centrifuge tube. Put in 1 steel ball with a diameter of 5 mm, and add 500 μL of 2% CTAB extraction buffer, cover the centrifuge tube and...

Embodiment 2

[0026] The SSR identification method for the seed purity of Chinese cabbage hybrid Yuxin 55, including the steps of material preparation, DNA extraction, genome template stock solution preparation, primer screening and result analysis, the SSR identification method for the seed purity of Chinese cabbage hybrid Yuxin 55, Specific steps are as follows:

[0027] S1: Material preparation: 20 copies of Yuxin 55 Chinese cabbage hybrids were randomly sampled in the field, and SSR primers were artificially synthesized based on the published EST sequence information of Chinese cabbage;

[0028] S2: DNA extraction: Use the young leaves on a single Chinese cabbage plant to extract the genomic DNA of Chinese cabbage. When extracting genomic DNA, use a centrifuge tube to buckle 4 young leaf disks the size of the centrifuge tube cap, and pour them into each centrifuge tube. Put in 1 steel ball with a diameter of 5 mm, and add 700 μL of 2% CTAB extraction buffer, cover the centrifuge tube an...

Embodiment 3

[0033] The SSR identification method for the seed purity of Chinese cabbage hybrid Yuxin 55, including the steps of material preparation, DNA extraction, genome template stock solution preparation, primer screening and result analysis, the SSR identification method for the seed purity of Chinese cabbage hybrid Yuxin 55, Specific steps are as follows:

[0034] S1: Material preparation: 15 copies of Yuxin 55 Chinese cabbage hybrids were randomly sampled in the field, and SSR primers were artificially synthesized through the published EST sequence information of Chinese cabbage;

[0035] S2: DNA extraction: Use the young leaves on a single Chinese cabbage plant to extract the genomic DNA of Chinese cabbage. When extracting genomic DNA, use a centrifuge tube to buckle 4 young leaf disks the size of the centrifuge tube cap, and pour them into each centrifuge tube. Put in 1 steel ball with a diameter of 5 mm, and add 600 μL of 2% CTAB extraction buffer, cover the centrifuge tube and...

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Abstract

The invention discloses an SSR identification method of purity of Chinese cabbage hybrid Yuxin No.55 seeds, and belongs to the technical field of vegetable breeding. The SSR identification method comprises steps of material preparation, DNA extraction, genome template stock solution preparation, primer screening and result analysis. Specifically, the SSR identification method of purity of the Chinese cabbage hybrid Yuxin No.55 seeds comprises the following steps : S1: material preparation; S2: DNA extraction; S3: genome template stock solution preparation; S4: primer screening; and S5: result analysis. With the application of the method, genotypes, which are different or even quite close in genetic relationship, can be effectively identified; as a relatively ideal method in the aspect of hybrid seed purity identification, the method has the advantages of being accurate, rapid and co-dominant, easy for analysis and reliable in analysis result; PCR amplification products can be separated just by conducting PCR amplification once in the identification of the hybrid seed purity, so as to achieve large-scale detection conveniently; and meanwhile, the identification method, which is not affected by weather factors, is quite strong in practicability.

Description

technical field [0001] The invention relates to the technical field of vegetable breeding, in particular to an SSR identification method for the purity of Chinese cabbage hybrid Yuxin 55 seeds. Background technique [0002] Chinese cabbage is a vegetable native to China, also known as "heading cabbage", "wrapped cabbage", "huangyabai", "jiaocai", etc. It is called "Shaocai" in Cantonese. Chinese cabbage and Chinese cabbage are close relatives, both belong to the Brassica species, and are also relatively close to the cabbage native to the Mediterranean coast, and both belong to the Brassicaceae Brassica genus. Cabbage is native to northern my country and is a leafy vegetable of the genus Brassica in the family Brassicaceae, usually referring to Chinese cabbage. Introduced to the south, it is cultivated in all parts of the north and south. In the nineteenth century, it was introduced to Japan, Europe and the United States. There are many kinds of Chinese cabbage. The northe...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 张晓伟原玉香魏小春姚秋菊赵艳艳王志勇蒋武生杨双娟
Owner 河南省农业科学院园艺研究所
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