The preparation method of dalfonpyridine
A technology for pyridine and amino resin, which is applied in the field of preparation of 4-aminopyridine, can solve the problems of medium yield and is not suitable for industrial production, and achieves the effects of mild synthesis conditions, environmental friendliness and high product purity
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Embodiment 1
[0029] The preparation of embodiment 1 isonicotinic acid amide resin
[0030]
[0031] Weigh 10 g of Rink Amide-AM resin with a substitution degree of 3.0 mmol / g, add it to a solid-phase reaction column, wash it twice with DMF, swell the resin with DMF for 30 minutes, deprotect DBLK for 6 minutes + 8 minutes, and wash it with DMF 6 times . Weigh 7.38g (60mmol) isonicotinic acid and 8.9g (66mmol) HOBT dissolved in DMF, add 12mL (72mmol) DIPCDI under ice-water bath to activate for 3min, then add the mixed solution into the reaction column, react at room temperature for 2 hours, and indene Triketone detection reaction end point (if the resin is colorless and transparent, stop the reaction; if the resin develops color, extend the reaction for 1 hour). After the reaction was completed, the resin was washed with DMF for 3 times, and methanol shrunk to obtain 13.5 g of isonicotinic acid amide resin with a yield of 99%.
Embodiment 2
[0032] The preparation of embodiment 2 isonicotinic acid amide
[0033]
[0034] Add 13.5 grams of the peptide resin obtained in Example 1 into a 250mL single-necked bottle, add pre-configured TFA:H 2 O: 150 mL of a mixed solution of DCM=90:2:8, react at room temperature for 2 hours, filter the resin, and collect the filtrate. The resin was washed with a small amount of TFA, and the filtrates were combined. The filtrate was slowly added to 1500ml glacial ether for precipitation. Centrifuged, washed 5 times with glacial ether, and dried under reduced pressure to obtain 3.5 g of isonicotinic acid amide with a yield of 97% and a purity of 96% by HPLC.
Embodiment 3
[0035] The preparation of embodiment 3 isonicotinic acid amide
[0036] Add 13.5 g of the peptide resin obtained in Example 1 into a 250 mL single-necked bottle, add 150 mL of a pre-prepared TFA:DCM=90:10 mixed solution, react at room temperature for 2 hours, filter the resin, and collect the filtrate. The resin was washed with a small amount of TFA, and the filtrates were combined. The filtrate was slowly added to 1500ml glacial ether for precipitation. Centrifuged, washed 5 times with glacial ether, and dried under reduced pressure to obtain 3.0 g of isonicotinic acid amide with a yield of 90% and a purity of 96% by HPLC.
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