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Kit for guiding folic acid taking dose for pregnant women and using method of kit

The technology of a kit and detection kit is applied in the biological field to achieve the effect of reducing spontaneous abortion

Inactive Publication Date: 2017-04-19
成都睿杰森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the prior art lacks a test kit for quickly and accurately instructing the dosage of folic acid

Method used

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  • Kit for guiding folic acid taking dose for pregnant women and using method of kit
  • Kit for guiding folic acid taking dose for pregnant women and using method of kit
  • Kit for guiding folic acid taking dose for pregnant women and using method of kit

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) First collect 2ml of peripheral blood from pregnant women, anticoagulate with EDTA, and use SnoMag TM Blood DNA Extraction Kit extracts genomic DNA, and operates in strict accordance with the kit instructions.

[0034] (2) The genotypes of MTHFR C677T, A1298C and MTRR A66G were detected by HRM method. Direct application of LightCycler 480Real-Time PCR System for PCR amplification and HRM analysis. The total PCR reaction system is 10ul, including: genomic DNA 1ul, SsoFast EvaGreen Supermix 5ul, primer (F / R 10uMol) 0.5ul, ddH 2 O added to 10ul. The PCR reaction conditions were: 95°C for 10 min, 95°C for 30S, 60°C for 30S, 72°C for 20S, 45 cycles. HRM analysis conditions: 95°C for 1min, 40°C for 1min, 60°C for 1min, and then the melting curve data was collected from 60°C to 90°C at a rate of 0.1°C / s. The primer sequences are as follows:

[0035] MTHFR C667T:

[0036] F: 5'-GAAGCAGGGAGCTTTGAGGCTGACCTG-3'

[0037] R: 5'-TGCCTTCACAAAGCGGAAGAATGTGTCAGC-3'

[0038]...

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Abstract

The invention provides a kit for guiding a folic acid taking dose for pregnant women. The kit consists of MTHFR C667T, MTHFR A1298C and MTRR A66G amplification primers and a PCR reaction reagent. The invention also provides a method of using the kit. According to the kit provided by the invention, folic acid metabolism-related genes of the pregnant women are detected by virtue of an HRM method, and then, folic acid supplementing and taking are guided for the pregnant women in accordance with genotype, so that the pregnant women can safely and effectively take folic acid and the occurrence of such circumstances as spontaneous abortion, premature delivery, neural tube defects and the like can be relieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for instructing pregnant women to take folic acid and a use method thereof. Background technique [0002] Folic acid is a water-soluble B vitamin, and the demand for folic acid by pregnant women is 4 times higher than that of normal people. The first trimester is a critical period for fetal organ system differentiation and placenta formation, when cells grow and divide vigorously. Folic acid deficiency at this time can lead to fetal malformations. For example, neural tube defects with an incidence rate of about 3.8‰ in China include anencephaly and spina bifida. In addition, it may cause early spontaneous abortion. In the second and third trimesters, in addition to fetal growth and development, the mother's blood volume, breast, and placental development greatly increase the need for folic acid. [0003] Insufficient folic acid, pregnant women are prone to placen...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 喻堃
Owner 成都睿杰森生物科技有限公司
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