Preparation method of ginkgo leaf extract and determination method of effective components

Abstract

The invention discloses a preparation method of a ginkgo leaf extract, wherein the preparation method comprises the steps: preparing 100 representative ginkgo leaves from each plant at different periods, drying and crushing, optimizing an extract system composed of factors of the particle size, the solvent type, the solvent concentration, the solid-liquid ratio, the ultrasonic extraction power, the temperature and the extraction time by an orthogonal design or response surface method, extracting, leaching, eluting, and carrying out vacuum reduced pressure recovery of a solvent, to obtain a crude extract; then carrying out double-distilled water solution centrifugation, to obtain a macroporous resin adsorbed purified material, and dividing the macroporous resin adsorbed purified material into three parts, wherein a first part is ginkgo leaf extract ginkgo flavonoids compounds, a second part is ginkgo leaf extract ginkgo terpene lactone compounds, and a third part is ginkgo phenolic acid compounds. The invention also provides a determination method of the effective components of the ginkgo leaf extract, and the ginkgo leaf extract ginkgo flavonoid compounds, the ginkgo terpene lactone compounds and the ginkgo phenolic acid compounds are detected by mobile phase dissolving RP-HPLC.

Description

technical field [0001] The invention relates to a preparation method of ginkgo biloba extract and a method for determining effective components thereof. Background technique [0002] At present, in the prior art, the Ginkgo biloba extract cannot be prepared and measured during the Ginkgo cultivation process, resulting in the inability to effectively manage the Ginkgo biloba preparation. Contents of the invention [0003] The invention provides a preparation method of ginkgo leaf extract and a method for measuring effective components thereof, which can effectively manage the cultivation of ginkgo. [0004] The present invention has adopted following technical scheme: a kind of preparation method of Ginkgo biloba extract is characterized in that it comprises the following steps: [0005] Prepare 100 representative ginkgo leaves per plant in different periods, dry and crush them, and optimize the particle size, solvent type, solvent concentration, solid-liquid ratio, ultras...

AI Technical Summary

Problems solved by technology

[0002] At present, in the prior art, the Ginkgo biloba extract cannot be prepared and measured during the cultivation process of Ginkgo biloba, resulting in the inability to effectively manage the preparation of Ginkgo biloba.