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A method for establishing a liver fibrosis model induced by hyperhomocysteinemia

A technology for cysteinemia and liver fibrosis, applied in the biological field, can solve problems such as unclear expression of liver fibrosis

Active Publication Date: 2021-01-15
唐博
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently reported ubiquitin hydrolase 22 is mostly related to tumor malignancy, proliferation, metastasis and prognosis, but its expression in liver fibrosis is still unclear

Method used

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  • A method for establishing a liver fibrosis model induced by hyperhomocysteinemia
  • A method for establishing a liver fibrosis model induced by hyperhomocysteinemia
  • A method for establishing a liver fibrosis model induced by hyperhomocysteinemia

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Embodiment Construction

[0028] The implementation of the present invention will be described in detail below with examples, so as to fully understand and implement the implementation process of how the present invention uses technical means to solve technical problems and achieve technical effects.

[0029] The present invention provides a method for establishing a liver fibrosis model induced by hyperhomocysteinemia, comprising the following steps:

[0030] Step 1. Preparation of hyperhomocysteinemia model induced by high methionine diet in vivo:

[0031] Step 1.1, such as figure 1 As shown, 20 C57BL / 6 mice aged 6-8 weeks were selected and randomly divided into 2 groups, 10 mice in each group. One group was fed with normal feed (normal mouse liver tissue), and one group was fed with Content is 2% high methionine feed (containing enough B vitamins) (modeling group), after 2 weeks, put to death mice, inferior vena cava blood sampling, collect the blood detection homocysteine ​​level of mice, collect ...

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Abstract

The invention discloses a method for establishing a hepatic fibrosis model through hyperhomocysteinemia induction and belongs to the field of hepatic disease research in medical science. According to the method, hyperhomocysteinemia is established through oral medication of methionine which is conducted at present, so that the mouse hepatic fibrosis model is established through induction; and then, homocysteine in blood is measured, measurement is conducted through a western blot method, masson staining conducted on tissues, and identification is conducted through an immunohistochemical method. The mouse hepatic fibrosis model established through the method is easy to operate, short in cycle and high in success rate. By means of establishment of the fibrosis model, a reliable animal model is provided for deeply researching pathogenesis of fibrosis, preliminarily screening various treatment drugs and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a liver fibrosis model induced by hyperhomocysteinemia. Background technique [0002] Hcy is a sulfur-containing amino acid, an intermediate product of methionine metabolism, and participates in the key link of methionine cycle. Hyperhomocysteinemia is formed when HCY in the blood rises to a certain level (usually >16uM). The clinical manifestations of hyperhomocysteinemia are diverse, including fatty liver and liver fibrosis. [0003] Liver fibrosis is a serious disease worldwide. It is caused by abnormal growth of connective tissue in the liver leading to damage to liver cells. Pathological factors include viral hepatitis, alcoholism, immune damage, extracellular matrix, etc. In the stage of liver cirrhosis, the liver function damage caused by massive extracellular matrix (ECM) deposition is irreversible. The pathological process of liv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A61K31/7088A23K50/50A23K20/142
CPCA01K67/02A61K31/7088
Inventor 唐博李全忠齐广莹梁兴思蔡雄王林青
Owner 唐博
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