Application of domperidone in preparation of medicine for treating swine Japanese encephalitis
A technology of porcine Japanese encephalitis and domperidone, which is applied in the field of application of domperidone for the preparation of porcine Japanese encephalitis therapeutic drugs, and can solve the problems of lack of porcine Japanese encephalitis and unobvious curative effect of porcine Japanese encephalitis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0022] This example mainly illustrates the specificity and dose dependence of domperidone in resisting JE virus-induced CPE and inhibiting JE virus replication at the cellular level.
[0023] Materials and methods:
[0024] 1. Toxicity testing. Plate the BHK21 cells on a 96-well cell culture plate, replace 80%-90% of the BHK21 cells with a maintenance solution containing 0.7% fetal bovine serum, and add different concentrations of domperidone. , 8, and 16 μM in six concentration gradients, and set up three duplicate wells for each concentration, and set up a negative control group of BHK21 cells, and culture at 37°C for 3 days. Cell Counting Kit-8 (DoJinDo product) was used to measure the degree of cytopathic disease (expressed as OD450) on a microplate reader (BIO-RAD product, model: Model 680) to observe the influence of domperidone on the cells.
[0025] 2. Specific verification of drug efficacy. Plate the BHK21 cells on a 96-well cell culture plate, replace 80%-90% of t...
Embodiment 2
[0036] This example mainly illustrates the evaluation of the anti-JEV activity of domperidone in mice.
[0037] Materials and Methods
[0038] Order a number of SPF grade three-week-old BALB / C female mice. At the beginning of the experiment, the mice are weighed and grouped in advance. Prepare the experimental equipment: 1mL syringe, cotton swab, electronic scale, and record book. During the experiment, both the PBS control group and the JEV virus infection group were injected with a certain amount of liquid in one side of the abdominal cavity according to their body weight for five consecutive days. In the following four days, only the same dose of domperidone was injected, followed by daily observation and weighing for recording, and continued observation for 20 days.
[0039] result:
[0040] The mice in the virus infection group died from the 7th day, and all the mice in the virus infection group died on the 14th day, and the survival rate of the mice in the domperidone...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More