Preparation method of spirulina platensis protoplasts

Abstract

The invention relates to a method for obtaining spirulina platensis protoplasts through spirulina platensis wall elimination. According to the method, 20ml of a spirulina platensis culture solution cultured to the mid-log phase OD<560>=0.800-1.000 is taken; about 30 to 60 glass beads for 3 to 10mm are put into the culture solution; rocking rotation is performed for 18h in a spirulina platensis constant temperature illumination culture box under the conditions of the rotating speed being 100 to 180 rpm and the temperature being 28 DEG C; the materials are subjected to split charging to enter a centrifuge tube; centrifugation is performed for 10 min at 4000 rpm / min and 4 DEG C; spirulina platensis segments are collected; washing is performed for 5 min by 15ml of 1.5mol / L NaCl; flushing is performed by a Zarrouk liquid culture medium; and centrifugation is repeatedly performed for 3 to 4 times; an enzyme solution is added; enzymolysis is performed under the dark condition for 1h at the enzymolysis temperature of 28 DEG C; after the enzymolysis is completed, centrifugation is performed for collecting precipitates; and the spirulina platensis protoplasts are obtained. The obtained spirulina platensis protoplasts reach 25 percent to 50 percent. The invention provides the method for preparing the spirulina platensis FACHB904 protoplasts with the advantages that the yield is high; the operation method is simple; and the consumed time is short.

Description

technical field [0001] The invention relates to a method for preparing spirulina protoplasts, belonging to the field of biotechnology. Background technique [0002] Spirulina (Spirulina platensis) belongs to cyanobacteria. Spirulina, also known as cyanobacteria, is dark green. Modern nutritionists call it "the miniature treasure house of human nutrition". Spirulina contains a variety of nutrients and has great nutritional and medical care It has been praised by the World Food and Agriculture Organization (FAO) as the most ideal food for human beings in the 21st century. The protein mass fraction in spirulina is as high as 55%-70%, rich in as many as 18 kinds of amino acids, which have the functions of improving the body's immunity and inhibiting cancer cells, and are easily absorbed by the human body. [0003] In recent years, the development of spirulina has attracted the attention of many scientific researchers. Spirulina contains a lot of protein, but at this stage, most...

AI Technical Summary

Problems solved by technology

[0006] The above method, or the yield is not reported, or the operation method is relatively cumbersome, and the delay is long

In short, although some work has been done on the preparation of Spirulina platensis protoplasts at home and abroad, so far, it has not been possible to reliably prepare Spirulina protoplasts with high quality and high yield.

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