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Tilmicosin phosphate immune colloidal gold detection card and preparation method thereof

A technology of tilmicosin phosphate and colloidal gold, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple method and accurate results

Inactive Publication Date: 2017-04-26
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, the purpose of the present invention is to overcome the defects of the prior art, and provide a kind of immune colloidal gold detection card and preparation method of tilmicosin phosphate, which can effectively solve the problem of rapid and effective detection of the antibiotic tilmicosin phosphate. test star question

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  • Tilmicosin phosphate immune colloidal gold detection card and preparation method thereof
  • Tilmicosin phosphate immune colloidal gold detection card and preparation method thereof

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Effect test

Embodiment 1

[0019] figure 1 It is the structural diagram of the immune colloidal gold detection card of tilmicosin phosphate of the present invention, figure 2 It is the sectional structural diagram of the test strip in the immune colloidal gold detection card of tilmicosin phosphate of the present invention: among the figure 9 is a PVC rubber sheet; 7 is a sample pad; 8 is a colloidal gold bonding pad, and the colloidal gold bonding pad is covered with was coated with monoclonal anti-soil colloidal gold marker; 10 is the coated membrane, that is, nitrocellulose membrane, which is coated with tilmicosin phosphate-BSA and goat anti-mouse IgG; Made of materials such as filter paper.

[0020] Adhere sample pad 7, colloidal gold bonding pad 8, sample pad 7 and glue on the other end (sample end) of PVC rubber plate 9

[0021] The bulk gold bonding pads 8 are in a side-by-side structure.

[0022] A nitrocellulose membrane 10 is adhered in the middle of the PVC colloid 9, and a goat anti-mou...

example 2

[0025] The preparation of colloidal gold detection card for tilmicosin phosphate is realized by the following steps:

[0026] (1) Preparation of gelatinous gold solution: Take a 1 L Erlenmeyer flask, add 495 mL of ultrapure water, and then add 5 mL of 1% chloroauric acid to prepare 500 mL of 0.01% chloroauric acid aqueous solution, heat to boil and then continue stirring Add 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) solution 5-7mL, continue to stir and heat, when the color of the solution turns into transparent purple red completely, stop heating after maintaining 5min, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0027] (2) Antibody pretreatment: centrifuge the tilmicosin phosphate antibody to be labeled at 1000 r / min, 4°C for 20 minutes, take the supernatant, and dilute it with 0.01mol / L PBS to 1mg / mL; or use 0.0 1mol / L PBS was diluted to 1mg / mL, and passed through a 0.22 um filter membrane;

[0028] (3) Preparati...

Embodiment 3

[0034] Tilmicosin Phosphate Minimum Detection Limit Test

[0035] Accurately weigh 0.500g of tilmicosin phosphate standard, dissolve it in ultrapure water and dilute to 100mL, marked as A 1 solution, take A 1 Add 1 mL of solution to a volumetric flask and add ultrapure water to 1000 mL to form A 2 solution, take A 2 Add 1mL of the solution to a volumetric flask and add ultrapure water to 1000mL to form A 3 solution, then A 3 The concentration of the solution was 5 ng / mL (5 ppb). take A 3Put 1mL of the solution in 4 test tubes, and label them 1~4 respectively, add 9mL, 4mL, 1ml, 0 ml, ultrapure water to the test tubes respectively, and prepare the tilmicosin A phosphate standard solution. The solubility of the solution is: 0.5ng / mL.1ng / mL. 2.5ng / mL, 5ng / mL; take another empty test tube, add 5mL ultrapure water, and mark it as No. 5 test tube. Take the liquid in the above test tubes 1-5 as a sample and add it dropwise to the tilmicosin phosphate test card, and observe the...

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Abstract

The invention provides a tilmicosin phosphate immune colloidal gold detection card and a preparation method thereof, and relates to the technical field of detection of antibiotics. According to the present invention, the tilmicosin phosphate immune colloidal gold detection card comprises a PVC glue plate, a sample pad, a colloidal gold conjugation pad, a coating membrane and a water absorbing pad, wherein the colloidal gold membrane is a glass cellulose membrane containing tilmicosin phosphate monoclonal antibody, the coating membrane is a nitrocellulose membrane and is provided with a T line and a C line, a tilmicosin phosphate protein conjugate is coated on the T line, and a goat anti-mouse IgG antibody is coated on the C line; and the detection card is effectively used for tilmicosin phosphate detection, and has advantages of convenience, rapidness, and accurate result.

Description

technical field [0001] The invention can be effectively used for determining the antibiotic tilmicosin phosphate, and the method is simple, convenient, fast and the result is accurate. Background technique [0002] Tilmicosin phosphate has similar antibacterial activity to macrolides, and is effective against Gram-positive bacteria, some Gram-negative bacteria and mycoplasma, and its pharmacodynamic antibacterial effect is similar to that of tylosin. It is also effective against gram-positive bacteria and a small number of gram-negative bacteria and mycoplasma. Its activity against Actinobacillus pleuropneumoniae, Pasteurella and Mycoplasma is stronger than Tylosin. It is reported that 95% of Pasteurella hemolytica strains are sensitive to this product. It is mainly used to prevent bovine pneumonia and mastitis caused by sensitive bacteria, and it is also used for mycoplasma disease in pigs and chickens. In recent years, the pollution of livestock products and the residue...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 洪霞刘静张淑雅
Owner JIANGSU WISE SCI & TECH DEV
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